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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2007, Vol. 12 ›› Issue (11): 1250-1254.

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Immunoreactivity and neutralization against king cobra venom of antiking cobra venom IgY

WANG Gui-ping1,2, LIU Xin-yan3, ZHU Liu3, YU Qing-sheng3   

  1. 1Nursing School,Guangzhou Medical College,Guangzhou 510182,Guangdong,China;
    2Institute of Gene Research,Southern Medical University,Guangzhou 510515,Guangdong,China;
    3Drug Research Center,Guangzhou Medical College,Guangzhou 510180,Guangdong,China
  • Received:2007-08-22 Revised:2007-10-29 Published:2020-11-04

Abstract: AIM: To explore the immunoreactivity and neutralization against king cobra venom of anti-king cobra venom IgY. METHODS: The anti-king cobra venom IgY was developed by thiophilic adsorption chromatography. The biological activity of anti-king cobra venom IgY was detected by indirect ELISA. In vivo and in vitro protective experiments in mice were used for evaluating the neutralization against king cobra venom of anti-king cobra venom IgY. RESULTS: The anti-king cobra venom IgY developed by thiophilic adsorption chromatography showed good biological activity and immunoreactivity,and it also neutralized king cobra venom well. A dose of 6 mg/kg anti-king cobra venom IgY could neutralize 4 LD50(about 1.6 mg/kg)of king cobra venom in vitro,and neutralize 3 LD50(about 1.2 mg/kg)of king cobra venom in vivo. CONCLUSION: The anti-king cobra venom IgY can neutralize king cobra venom,and the present study provides experiment data for further study of antiking cobra venom IgY.

Key words: king cobra venom, egg yolk immunoglobulin, immunoreactivity, neutralization

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