Effects of ozone-stress on E-cadherin expression in human bronchial epithelial cells

Abstract

Abstract: AIM: To explore the expression of Ecadherin(E-cd)in the human bronchial epithelial cells(HBECs)and their role in the damage and injury of the airway.METHODS: The E-cd protein expression and the E-cd mRNA of HBECs were determined by immunocytochemistry analysisand RT-PTR analysis , respectively.RESULTS: Ozone-stress caused a decrease in the HBECs membrane E-cd expression and an increase the cytoplasmic E-cd expression (p<0.05).These changes returned to normal 32 h after the ozone-stress was withdrawn.O₃-stress had little effect on the expression of HBECs E-cd mRNA (p>0.05).CONCLUSION: Ecd is distributed on HBECs.O₃-stress decreases the membrane E-cd expression but increases the cytoplasmic E-cd expression.The change of E-cd expression may mediate HBECs injuries induced by O₃-stress.

Key words: epithelial cadherin, human bronchial epithelial cells, ozone

CLC Number:

BAI Hong-bo<, GUAN Cha-xiang<, LIU Yong-ping<, ZHANG Min<, ZHENG Shi-ping<, CUI Yan-ru<, LIU Hui-jun. Effects of ozone-stress on E-cadherin expression in human bronchial epithelial cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(9): 999-1002.

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