Inhibitive effect of mistletoe lectins on cyclooxygenase-2 expression in colon cancer HCT116 cell line

Abstract

Abstract: AIM:To investigate the effect of mistletoe lectins on cyclooxygenase-2 (COX-2) expression in colon cancer HCT116 cells at the level of gene and protein.METHODS: Levels of mRNA and protein expression of COX-2 and its synthesized product prostaglandin E₂ (PGE₂) of colon cancer HCT116 cell line were detected by RT-PCR, Western blotting, ELISA respectively before and after treatment of mistletoe lectins in different concent rations. RESULTS:Mistletoe lectins had a remarkable inhibitory effect on the mRNA and protein expression of COX-2, and synthesis of PGE₂ in colon cancer HCT116 cell line, but had effect on COX-1 only at large dose. CONCLUSION:Mistletoe lectins has selective inhibitory effect on gene transcription, protein expression and functional activity of COX-2 in HCT116 cell line, which is time-dependent and concentration-dependent within certain range of concentration and acting time.

Key words: mistletoe lectins, HCT116 cell line, cyclooxygenase-2, prostaglandin E₂

CLC Number:

R965. 2

LIN Lei, YE Meng, WANG Shao-ming, NI Shu-min, WU Xiao. Inhibitive effect of mistletoe lectins on cyclooxygenase-2 expression in colon cancer HCT116 cell line[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(5): 504-507.

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