Effects of crocin on apoptosis of vascular endothelial cells induced by triol

Abstract

Abstract: AIM: To investigate effects of Crocin on apoptosis of vascular endothelial cells injured by triol. METHODS: Apoptosis model induced by triol (cholestane-3β, 5α, 6β-triol) was adopted. Malondialdehyde (MDA) content was measured by colorimetric assay. The morphology and ultrastructure were detected by light microscope and electron microscope. DNA ladder was analyzed by DNA electrophoresis. The apoptosis rate was assessed with the flow cytometer assay. Bax mRNA expression of endothelial cells was measured by RT-PCR analysis. RESULTS: In Triol group, MDA content was 1.761 nmol·L^-1 (P <0.01). BAECs was shrinkage. The nucleus concentrated and dye deeply, mitochondria swelling and cavitations, mitochondrial cristae fragmentation, formation of apoptotic bodies, the DNA ladder in agarose gel and hypodiploid cusp was observed. Apoptosis rate was 30.62 %. Bax mRNA expression increased. In the Crocin +Triol group, MDA content decreased, majority of cells grown in spreading out like that in control and cholesterol group. Apoptosis rate of Crocin (10^-7 or 10^-6 mol·L^-1) +Triol group were 24.4 %, 6.3 %, respectively. Bax mRNA expression of endothelial cells in the crocin (10^-6 mol·L^-1) +Triol group decreased. CONCLUSION: Crocin maybe abnormally decrease apoptosis by anti-lipid peroxidation and adjust Bax mRNA expression.

Key words: crocin, blood vessel endothelial cells, triol, apoptosis, Bax, gene

CLC Number:

R966

LIU Juan, QIAN Zhi-yu. Effects of crocin on apoptosis of vascular endothelial cells induced by triol[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(6): 627-632.

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