Effects of selenium and Vitamin E in combination and separateness on lipid peroxidation and NO contents of hearts, livers, kidneys and serum in rats with hyperlipidemia

Abstract

Abstract: AIM: To investigate the effects of selenium (Se)and vitamin E (VE)in combination and separateness on the peroxidation of hearts, livers, kidneys and serum in rats with hyperlipidemia.METHODS: Highfat- diet (HFD)was used to cause experimental hyperlipidemia rats.50 SD rats were divided into 5 groups:control group ;high-fat-diet (HFD)group ;HFD +0.4 mg·kg^-1Se ;HFD +250 mg·kg^-1 VE ;HFD +0.4 mg·kg^-1 Se⁺250 mg·kg^-1 VE, respectively.After 4 weeks, the MDA contents in hearts, livers, kidneys and serum were measured through thiobarbituric acid (TBA)method.The NO contents in these samples were assayed by NO kits.RESULTS: HFD caused hyperlipidemia, in comparison with the control group, triglyceride (TG)and very low density lipoprotein (VLDL-C)increased(P<0.05), so did total cholesterol and low density lipoprotein (P<0.01). Meanwhile, HFD strengthened the peroxidation in all of the tissues or serum (P<0.05).NO contents were reduced in hearts and livers, but increased in serum and kidneys by HFD.VE or Se in combination and separateness did fight against these effects (P<0.05 or P<0.01).Meanwhile, VE and or Se can increase the NO contents in all the experimental tissues, combined use of Se and VE are more effective.CONCLUSION: Se and or VE can decrease the peroxidation-related damage and modify the NO contents in experimental hyperlipidemia rats.

Key words: selenium, Vitamin E, hyperlipidemia, peroxidation, MDA, nitric oxide(NO)

CLC Number:

R965
R977.25

ZHOU Chun-Yang, RAN Li, ZHANG Xiang, XU Bin, CAI Chun-Yan, FAN Ping. Effects of selenium and Vitamin E in combination and separateness on lipid peroxidation and NO contents of hearts, livers, kidneys and serum in rats with hyperlipidemia[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(2): 212-215.

References

1 张在香, 杨晓光, 夏弈明, 等.哺乳动物硒蛋白的研究进展[J]. 生理科学进展, 1998 ;29(1):27-34
2 宋晓燕, 杨天奎.天然维生素E 的功能及应用[J]. 中国油脂, 2000;25(6):45-7
3 Konopacka M, Rzeszowska-Wolny J.Antioxidant vitamin C, E and Beta-carotene reduce DNA damage before as well as aftergamma-ray irradiation of human lymphocytes in vitro[J]. Mutat Res, 2001;491:1-7
4 Reis EA, Zugno AI, Franzon R, et al. Pretreatment with vitamin E and C prevent the impairment of memory caused by homocysteine administration in rats[J]. Metab Brain Dis, 2002;17: 211-7
5 李玉华, 徐仓宝.硒、维生素E 等抑制低密度脂蛋白氧化修饰作用的比较研究[J]. 西安医科大学学报, 2001 ;22(2):97-99, 125
6 庞战军, 周玫, 陈瑗.自由基医学研究[M]. 第1 版.北京:人民卫生出版社, 2000:60-8
7 周春阳, 顾峥, 江明华.Kappa-硒化卡拉胶对老年大鼠心、肝、肾、血清脂质过氧化水平的影响[J]. 四川生理科学杂志, 1999 ;21(1):36-40
8 Cuzzocrea S, Riley DP, Caputi AP, et al. Antioxidant therapy:a new pharmacological approach in shock, inflammation, and ischemia reperfusion injury[J]. Pharmacol Rev, 2001;53:135-59
9 尹石华, 陈光连, 张才林.超氧化物歧化酶、丙二醛含量与脑血管病关系的研究[J]. 中国实验临床免疫学杂志,1997 ;9(4):32-7
10 Vassalle C, Lubrano V, L' Abbate A, et al. Determination of nitrite plus nitrate and malondialdehyde in human plasma:analytical performance and the effect of smoking and exercise[J]. Clin Chem LabMed, 2002;40:802-9
11 岳旭, 郭燕茹, 季凤清, 等.缺氧复氧时培养的大脑皮层细胞一氧化氮动态变化及银杏提取物的调节作用[J]. 神经解剖学杂志, 2002, 18(1):32-6
12 Wink DA, Michell JB.Chemical biology of nitric oxide:insight into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide[J]. Free Radic Biol Med, 1998 ;25:434-56
13 赵水平.家族性混合型高脂血症[J]. 中国动脉硬化杂志, 1996;4(4):304-7
14 Vadim N, Gladyshev, Thressa C, Stadtman, Dolph L, Hatfield, et al. Levels of major selenoproteins in T cells decrease during HIV infection and low molecular mass selenium compounds increase[J]. Proc Natl Sci USA, 1999 ;96(3):835-9
15 Poirier J, Cockell K, Hidiroglou N, et al. The effects of vitamin E and selenium intake on oxidative stress and plasma lipids in hamsters fed fish oil[J]. Lipids, 2002;37(12):1125-33
16 Scholz RW, Minicucci LA, Reddy CC.Effects of vitamin E and selenium on antioxidant defense in rat hearts[J]. Biochem Mol Biol Int, 1997 ;42(5):997-1006

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