Effects of 3 traditional Chinese formulas of herbal medicine inPrevention and treatment of diabetic rats induced by streptozotocin

Abstract

Abstract: AIM: To investigate the mechanisms of Chinese herbal medicine formulas(Qi-ling-tang,Ba-weidi-huang-wan and Chai-ling-tang)in thePrevention and treatment of diabetes.METHODS: Wistar rats were di-· 684· Chin J ClinPharmacol Ther 2003 Dec;8(6)vided into five groups and treated with Qi-ling-tang(the grouPQLT),Ba-wei-di-huang-wan(the grouPBWDHW),Chai-ling-tang(the grouPCLT),water(the grouPmodel)and control,respectively.The rats in groups QLT through model were injected with 30mg·kg^-1/daily streptozotocin(STZ)for five days beginning on day 8.The glucose concentration,fructosamine,insulin,total-protein,albumin and thePercentage ofW3/25 and OX8 T-cells in serum as well as the body weight of the rats were measured.Tissue sections ofParaffin-embeddedPancreas of the rats were observed after staining with HE,AZAN and immunohistochemistry.RESULTS: After STZ injection,the diabetic incidence of the rats in groups QLT,BWDHW and CLT were significantlylower than that in the grouPmodel,especially in the grouPBWDHW was only 21% by study day 10.A significant decrease in blood glucose concentration was found in groups BWDHW(25%)and CLT(18%)from study day 15 to 26.ThePercentage of W3/25 cells in groups QLT and BWDHW was higher than that in the control group.The average area of islets and the area of bate cells were greater in groups QLT,BWDHW and CLT than in the grouPmodel.CONCLUSION: Three formulas retard the development of hyperglycemia of rats induced with multiplelow doses STZ by increasing the cell mediated immunofunction.The formulas Ba-wei-di-huang-wan and Chailing-tang can reduce hyperglycemia.

Key words: pharmacodynamics, diabetic rats, streptozotocini, herbal medicine, cell-mediated immunity, immunohistochemistry

CLC Number:

R285.5

WEN Zhi-Ming. Effects of 3 traditional Chinese formulas of herbal medicine inPrevention and treatment of diabetic rats induced by streptozotocin[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2003, 8(6): 680-685.

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