Potential effect and mechanism of IFNγon tumor angiogenesis

Abstract

Abstract: AIM: To study the effect and potential mechanism of IFNγon tumor angiogenesis.METHODS: Human umbilical cord vascular endothelial cell (HUVEC) was cultured in vitro.The effect of IFNγ(10, 100, and 1000 unit·ml^-1)on proliferation of HUVEC was detected by MTT assay in vitro.HUVEC pretreated with IFNγ(10, 100, and 1000 unit·ml^-1)was stained with PI and detected by flow cytometry (FCM).The mRNA expression of apoptosis relative gene Bcl-2 and Bax of HUVEC treated with IFNγ(100, and 1 000 unit·ml^-1)were detected with semi quantitative reverse transcription-polymerase chain reaction (RT-PCR).IFNγ(30, 300, and 3 000 unit·ml^-1)on VEGF secretion and expression in human lung carcinoma cell PG in hypoxia were detected by ELISA assay.CONCLUSION: The key attributes of IFNγon tumor angiogenesis possible directly inhibit vascular endothelial cells or indirectly inhibit growth factors secretion and expression in tumor cells.

Key words: IFNγ, tumor angiogenesis, HUVEC, human lung carcinoma cell PG

CLC Number:

R979.1

CAO Qi-Zhen, LIN Zhi-Bin. Potential effect and mechanism of IFNγon tumor angiogenesis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(8): 863-867.

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