Inhibition of prodigiosin on multiplication of human malignant pancreas cancer cells

Abstract

Abstract: AIM: To investigate the inhibitive effects of prodigiosin on the multiplication of human malignant pancreas cancer cell 8898, and its mechanism. METHODS: MTT assay was used to measure the rate of viability and inhibition of 8898 cells;the change of cell cycle and the percent of apoptosis were analyzed by FCM;the concentration of prodigiosin in 8898 cells was measured HPLC;and the fragmented DNA was analyzed by agarose gel electrophoresis. RESULTS: In the culture medium containing 5 mg·L^-1 prodigiosin, 8898 cells appeared the morphological character of early stage of apoptosis.Half inhibition concentration (IC50) was 30 mg·L^-1.But to 3T3 cells the drug had no obvious effect.The inhibitive effect of prodigiosin on multiplication of cancer cells was related to the inhibiting of DNA replication in S stage and regulating of cell cycle.The results of HPLC showed that pharmacological effect of prodigiosin dependent on drug concentration in cells. CONCLUSION: Prodigiosin can enter cells and inhibit the multiplication of cells.The mechanism may be related to inducing apoptosis and regulating cell cycle.

Key words: prodigiosin, human pancreas cancer, apoptosis, proliferation cycle, FCM analytical method

CLC Number:

R285.5

SHEN Ya-Lin, LIU Jian-Wen, WEI Dong-zhi, TAO Jin-Li, LI Ke, ZHANG Jing. Inhibition of prodigiosin on multiplication of human malignant pancreas cancer cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(5): 504-509.

References

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