Arsenic trioxide-induced apoptosis in K562/ADM cells and its mechanisms

Abstract

Abstract: AIM: To observe the apoptosis of K562/ ADM cells induced by As2O3 and to explore its possible mechanisms. METHODS: MTT assay, Wright-Giemsa staining, DNA agarose gel electrophoresis and cell cycle analysis were used to examine apoptosis in K562/ADM cells.Expression levels of Fas, Bcl-2 and P53 antigens were measured with FCM.Colorimetric assay was employed to detect the activation of Caspase3. RESULTS: As2O3 inhibited growth of K562/ADM cells.Morphological changes typical of apoptosis were observed through light microscopy.Agarose gel electrophoresis showed evident DNA fragmentation.Cell cycle analysis indicated increased Sub-G1 proportion and apoptosis rate, as well as apparent G2 M phase arrest.The expression of Fas and P53 antigens significantly increased after application of As2O3.Caspase 3 was also activated by As2O3. CONCLUSION: As2O3 induces apoptosis in K562/ADM cells by activating Caspase 3 via a Fas-dependent pathway.

Key words: leukemia, multidrug resistance, arsenic trioxide, apoptosis

CLC Number:

WANG Dong-Hai, WEI Hu-Lai, SU Yong-Hong, HAO Chun-Yan, LIU Jian-Min. Arsenic trioxide-induced apoptosis in K562/ADM cells and its mechanisms[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(5): 569-572.

References

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