Reverse effects and its mechanisms of Xueyaping on left ventricle remodeling resulting from renal hypertension induced by 2K1C in rats

Abstract

Abstract: AIM: To investigate the reverse effects and its mechanisms of Xueyaping on left ventricle remodeling resulting from renal hypertension induced by 2K1C in rats (RHR).METHODS: Renal hypertension rat was induced by 2K1C.40 RHRs were randomly divided into 5 groups with 8 rats in each group 8 weeks after induction including:group 1 was the large dosage Xueyaping (73 g·kg^-1·d^-1) group, group 2 was the medium dosage Xueyaping (36.5 g·kg^-1·d^-1) group, group 3 was the low dosage Xueyaping (18.25 g·kg^-1·d^-1) group, group 4 was the enalapril group (10mg·kg^-1·d^-1), and group 5 was the model group.Besides, psydeo-operation was performed on another 8 rats and set as the control group.Iso-volume pure water was poured into stomach in group 4 and group 5 for 8 weeks.Blood pressure was taken before operation and once a week after operation.By the end of the eighth week, the body weight was measured and 2 ml blood was taken through eye sockets.The rats were then put to death by cuttign off their heads.The heart was removed rapidly and atrium and free wall of right ventricle were abounded.The left ventricle and interventricular septal weight (LVW) were measured. LVW/BW ratios were calculated.Serum PCIII was measured using radio-immune method.Paraffin embedded heart tissue was cut and stained with HE and SP immunohistochemistry.Conformation parameters and mean light density of positive c-myc and c-fos expression heart muscle cells were measured using high resolution picture analyzing system.RESULTS: Xueyaping had the effects of lowering blood pressure, decreasing LVW, LVW/BW, heart muscle cell volume, serum PCIII, and the expression of c-myc and c-fos.SBP, LVW, LVW/BW and serum PCIII in group 1 and 2 were significantly lower than those in group 5 (P <0.01).Conformation parameters of heart muscle cells (area, circle length, mean diameter, length of long and short axes) and mean light density of positive c-myc and c-fos expression cell in group 1 and 2 were significantly lower than those in group 5 (P < 0.01), and were close to those in the sham operation group (P >0.05).CONCLUSION: Xueyaping has the effects of reversing left ventricle remodeling in hypertensive rats induced by 2K1C.The effects may be related to the partial mechanisms of reversing left ventricle remodeling in hypertensive rats induced by 2K1C.

Key words: 2K1C renal hypertensive rat, left ventricle remodeling, serum PCIII, proto-oncogene

CLC Number:

R972.4

SHI Jie, HU You-zhi, QU Song-bai, XIANG Nan, KE Yu-he, FENG De-xun, LI Da-feng. Reverse effects and its mechanisms of Xueyaping on left ventricle remodeling resulting from renal hypertension induced by 2K1C in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(9): 1053-1058.

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