Inhibition of active fraction separated from Naja Atra venom on three-dimensional angiogenesis

Abstract

Abstract: AIM: To study the anti-angiogenesis effects of the active fraction separated from Naja Atra venom.METHODS: The inhibiting effect of active fraction in angiogenesis was detected by the three-dimensional angiogenesis model. RESULES: The active fraction in different concentrations inhibit the action that vascular endothelial cells (ECs) developed into three-dimensional capillary-like networks in vivo. ECs in matrigel only developed into incomplete capillary-like networks in 0. 6 μg·ml^-1 dose group, the sprouts differentiated from ECs did not spread and contract each other and form the nets in 1. 2mg·L^-1 dose group, ECs did not adherent together and alternate in 2. 4 mg·L^-1 dose group.CONCLUSION: The active fraction separated from Naja Atra venom has the anti-angiogenesis effect in in vitro.

Key words: Naja Atra venom, anti-angiogenesis, three-dimensional angiogenesis, endothelial cells (ECs), in vitro, cell culture

CLC Number:

R966

YU Qing-Sheng, LIU Xiao-Ying, QIU Yuan, HANG Shao. Inhibition of active fraction separated from Naja Atra venom on three-dimensional angiogenesis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(10): 1105-1109.

References

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