Abstract: AIM: To investigate the transdermic absorption and accumulation in vivo after dermic administration of 1 % liquor of terbinafini hydrochloridum.METHODS: 6 New Zealand white rabbits were used in this experiment. 8 ml of the liquor was coated on the back skin of each rabbit. Blood were collected at 0. 5, 1, 1. 5, 2, 3, 4, 6, 8, 12 and 24 h before and after single administration. Blood were collected at peak and trough points everyday in the continuous multiple administrations which was once per day for 7 days. The serum concentrations of terbinafine were determined by high-performance liquid chromatography.RESULTS: The peak concentration of terbinafine arrived at 2-3 h and C_max was 8. 6 ± 1. 8 μg·L^-1.The peak concentration of terbinafine increased gradually after continuous multiple administrations, but there only increased 1. 33 times in fourth day, and more than that in first day. In following three days, the peak concentrations tend to steady-state. Therewas no significantly increase or decrease in trough concentration.CONCLUSION: There is no accumulation in vivo after dermic administration of 1 % liquor of terbinafini hydrochloridum.

Key words: high-performance liquid chromatography, terbinafine, serum concentration, transdermic absorption, cumulation