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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2002, Vol. 7 ›› Issue (1): 48-50.

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Determination of etheofazine (EDMTP)and its metabolite in human plas-ma by reversed-phase HPLC

FU Liang-Qing, LIANG Yue-Qin, SUN Cheng-Chun1, LI Jie, WU De-Zheng   

  1. Department of Clinical Pharmacology, Accessary Hospital, Academy of Military Medical Sciences, Beijing 100039;
    1Department of Pharmacology, General Hospital of Jinan Military Command, Jinan 250031
  • Received:2001-10-09 Accepted:2001-10-29 Online:2002-02-26 Published:2020-11-27
  • About author:FU Liang-Qing, female, PhD

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Abstract: AIM: To develop a method to determin- ing etheofazine(EDMTP) and its metabolite etheotriazene (EDMTP-Ⅱ) by HPLC in human plasma.METHODS: Human plasma samples containing etheofazine(EDMTP) and its metabolite EDMTP-Ⅱ after addition of internal standard (IS) , was extracted with ethylacetate and sepa- rated in a reversed-phase ODS column, and the detection was achieved at 345 nm by ultra-violet (UV).RESULTS: The limit of detection was 0.05 mg·L-1 for both etheofazine(EDMTP) and its metabolite EDMTP-Ⅱ. No potential interference was found.The method provided recoveries up to 81.42%-104.67%and accepted coef- ficients of variation were found for both within-run (＜9.95%) and day-to-day (＜9.14%) assays.CONCLUSION: This method is rapid, sensitive and simple for studying the pharmacokinetics of EDMTP and its metabolite EDMTP-Ⅱ .

Key words: etheofazine, HPLC, pharmacokinetics

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