Abstract: Aim To evaluate the clinical significance of determination of serum or urine tumor necrosis factos (TNF)and whole blood concentration of cyclosporin A (CsA)in renal transplanted recipients.Methods The serum and urine TNF levels with radioimmunoassay and whole blood concentration of CsA with FIPA method were determined in 55 renal transplanted re-cipients.Results Serum and urine TNF levels in group of acute rejection (1.03±0.26 ng/ L, 0.44±0.36 ng/L)were significantly higher than those in group of functionally stable re-nal graft(0.86±0.21ng/L, 0.34±0.29 ng/L)and there was an obvious elevation of the TNF as early as 1～ 3 daysbefore the rise of serum creatine.But the TNF levels in CsA nephrotoxicity group (0.91±0.22 ng/L, 0.4±0.27 ng/L)were not significantly different from those in func-tionally stable renal graft group. The blood concentration of CsA was decreased in group of acute rejection and increased in group of CsA nephrotoxicity. Conclusion Determining TNF and CsA could distinguished the graft rejection from CsA nephrotoxocity. It has important clinical sig-nificance for regulation of the dosage of CsA.

Key words: cyclosporin A;tumor necrosis factor;transplantion/kidney