Effect of Pinaverium Bromide on different layers and segments of colonic smooth muscle strips in wrap restraint stress rats

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2006, Vol. 11 ›› Issue (3): 314-318.

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Effect of Pinaverium Bromide on different layers and segments of colonic smooth muscle strips in wrap restraint stress rats

LI Cui-ping, LIU Shi, XU San-ping, QIAN Wei, HOU Xiao-hua

Department of Gastroenterology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei,China

Received:2005-12-01 Revised:2006-01-21 Online:2006-03-26 Published:2020-12-04
Contact: LIU Shi,female,MD,associate professor,tutor of master,engaged in gast rointestinal motility.Tel:027-85726381 　E-mail:shiliugao@yahoo.com
About author:LI Cui-ping,female,MD,engaged in gastroint estinal motility.Tel:027-62420503 　E-mail:cuipingli76@yahoo.com.cn

Abstract

Abstract: AIM: To evaluate effects of Pinaverium Bromide on different segments and layers of colonic smooth muscle in wraPrestraint stress (WRS) rats and explore its possible therapeutic mechanism on different types of irritable bowel syndrome (IBS).METHODS: Adult SD rats were randomly divided into model group(wraPrestraint stress group) and control group.Colonic smooth muscle strips were made from different segments and layers in two groups.The spontaneous contraction activities of colonic longitudinal/circular muscle (LM/CM)strips of rats were observed with organ bath system before and after addition of series concentrations of pinaverium.RESULTS: Pinaverium Bromide caused concentrationdependent inhibition of colonic smooth muscle,the inhibitory effect of pinaverium in model grouPwas significantly stronger than that in control group(proximal colon:28.54±4.82 vs 7.48±1.65,21.75±1.00 vs 12.56±3.15;distal colon:15.71±5.27 vs 3.89±1.16,20.16±3.16 vs 7.56±1.96) (P<0.05).Compared with that of distal colon,inhibitory effect of pinaverium was significantly higher of proximal colon (P<0.05).For the inhibition of pinaverium,there was no significant difference between LM and CM strips in the same intestinal segments (P>0.05).CONCLUSION: Effects of Pinaverium Bromide on different colonic muscle layers and segments in WRS rats is probably related with its therapeutic mechanism on different types of IBS.

Key words: Pinaverium Bromide, wrap restraint stress, smooth muscle strip, colonic motility

CLC Number:

R965.2

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Effect of Pinaverium Bromide on different layers and segments of colonic smooth muscle strips in wrap restraint stress rats

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