Welcome to Chinese Journal of Clinical Pharmacology and Therapeutics,Today is Chinese

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2006, Vol. 11 ›› Issue (3): 324-327.

Previous Articles     Next Articles

Effect of anti-huamn TLR9 antibody on CpG tumor necrosis factor induced tumor necrosis factor-αrelease from human peripheral blood mononuclear cells

WU Chong, DING Guo-fu, ZHOU Hong   

  1. Departmen of Pharmacology,Third Military Medical University,Chongqing 400038,China
  • Received:2006-02-09 Revised:2006-03-07 Online:2006-03-26 Published:2020-12-04

Abstract: AIM: To observe the role of cell-surface TLR9 during TLR9-mediated signal transduction pathways.METHODS: Using anti-huamn TLR9 antibody to blockade cell-surface TLR9,internalization of CpG oligodeoxynucleotide(ODN) and tumor necrosis factor-α(TNF-α) release in human peripheral blood mononuclear cells (hPBMCs) were investigated.RESULTS: Both of CpG ODN and GpC ODN were endocytosed by the cells,only CpG ODN could induce TNF-αrelease.Addition of chloroquine (CQ),an inhibitor of endosome mature,delayed degradation of both ODN molecules,with no obvious difference between them.Importantly,blockade of cell-surface TLR9 using an anti-hTLR9 antibody did not significantly affect CpG ODN internalization,CpG ODNinduced TNF-αrelease,or CQ-induced inhibition of TNF-αrelease in hPBMCs.CONCLUSION: Cell-surface TLR9 does not directly participate in internalization of CpG ODN or TNF-αrelease from hPBMCs.

Key words: CpG ODN, TLR9, TNF-α, internalization, chloroquine

CLC Number: