Abstract: AIM: To investigate the effect of Shenqi fuzheng injection (SFI) on tumor immunity and its preliminary molecular mechanism. METHODS: The animal model of low glucose tumor microenvironment was established by B16-PKM2-OE; the level of interleukin-2(IL-2) and interferon-γ(IFN-γ), CD40L and transforming growth factor-β1(TGF-β1) were detected by ELISA kit; the expressions of glucose transporter-1 (Glut-1) and key enzymes of glycolysis ( HK, PFK and PK ) in CD4+T cells were detected by Western blot. RESULTS: The results of the kit showed that SFI could increase IL-2, IFN-γ and CD40L, and decreased TGF-β1 in tumor tissues. Western blot assay showed that SFI could promote the expression of Glut-1 protein on the surface of CD4+T cell membrane, and up-regulated the expression of key enzymes of glycolysis (HK, PFK1 and PK) in a dose-dependent manner. CONCLUSION: SFI can increase the expression levels of IL-2, IFN-γ, CD40L and decrease the expression level of TGF-β1 in tumor tissues. Also, it increases the number of activated CD4+T cells in tumor tissue and promotes the glycolysis of CD4+T cells, indicating that SFI can improve the tumor immune microenvironment.

Key words: tumor infiltrating lymphocytes, Shenqi fuzheng injection, low sugar microenvironment