Abstract: AIM: To investigate the protective effect of guanxinshu capsule on cardiomyocytes after anoxia-reoxygenation(A/R) injury, partial formulation experiment was executed by orthogonal design in order to obtain the best ratio of Guanxinshu.METHODS: Based on the effective site and the basic ratio isolated from original chemical formulation of salvia miltiorrhiza, panax pseudo-ginseng, lignum acronylchiae, orthogonal table was designed.The primarily neonatal rat ventricular cardiomyocytes were randomly divided into 18 groups: (1)control group; (2)A/R group; (3)-(18) experimental groups treated with different ratio; Cell viability, SOD, Catalase, GPx activities, MDA contents and LDH activitied were detected.Best ratio of program was obtained by analysis of integrated equilibrium and then verificated.RESULTS: These groups treated with different ratio of Guanxinshu elevated cell viability and SOD, Catalase, GPx activity, whereas LDH activities and MDA contents reduced(P<0.01 or P<0.05); Panax pseudo-ginseng saponins and salvia miltiorrhiza phenolic acid have played an important role of protection of cardiomyocyte and increased ability of attenuation lipid peroxidation; The best ratio of Guanxinshu is: A3B3C2D4.CONCLUSION: The different ratio of Guanxinshu have protected cardiomyocyte against A/R injury, the mechanism may be relative with attenuation lipid peroxidation.The best ratio of Guanxinshu is: salvia miltiorrhiza total phenolic acid 0.027 mg, Panax pseudo-ginseng total saponins 0.048 mg, lignum acronylchiae oil 0.002625 mg, lignum acronylchiae total flavonoids 0.01326 mg.

Key words: Guanxinshu, Cardiomyocyte, Anoxia-reoxygenation(A/R) injury, Orthogonal design, Partial formulation experiment