Acute toxicity of protein C activator from venom of Agkistrodon halys in rats and mice

Abstract

Abstract: AIM: To investigate the acute toxicity of protein C activator from venom of Agkistrodon halys in rats and mice.METHODS: Rats using the up-and-down procedure and mice using median lethal dose; the acute toxical symptoms, signs, death time and accumulated death rate were observed.RESULTS: PCA tail intravenous of rats (dose of 17.5-175 mg/kg), mice (dose of 6.25-11 mg/kg) poisoning in different degree of mouth and nose foam bleeding, dyspnea, heart rate, unstable walking, crouching, consecutive convulsion. The LD₅₀ and its 95%confident limit of PCA to rats and mice were 29.57 (17.5-55) mg/kg and 8.05 (7.55-8.54) mg/kg respectively. A massive hemorrhage was noted in double lung, and pathological examination shows hemorrhage mainly involved in the alveoli, bronchi, etc. Other organs showed no obvious abnormality.CONCLUSION: The main toxicity of PCA from venom of Agkistrodon halys was hemorrhage induced by anticoagulation. In addition, the acute respiratory failure induced by pneumorr- hagia was the major cause for animal death.

Key words: Protein C activator, Acute toxicity, Half lethal dosage

CLC Number:

DUAN Ting, ZHANG Gen-bao, ZHOU Bing. Acute toxicity of protein C activator from venom of Agkistrodon halys in rats and mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(1): 20-24.

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