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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2012, Vol. 17 ›› Issue (2): 137-140.

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Differentiation of rat bone marrow stromal cells into neurol cells induced by mouse nervous growth factor

CHEN Zai-feng, XU Xin-long, WEI Xiao-jie, FU Xiao-jun, PAN Hong-song, XIE Qin-song   

  1. Department of Neurosurgery, Cixi Municipal People's Hospital, Cixi 315300, Zhejiang, China
  • Received:2011-11-24 Revised:2012-01-13 Online:2012-02-26 Published:2012-03-12

Abstract: AIM: To observe the effect of mouse nervous growth factor(mNGF) on differentiation of rat bone marrow stromal cells into neurol cells.METHODS: 60 4-week-old healthy clean SD rats were equally randomized into divided into treatment group(n=30)and normal control group(n=30). The treatment group received 0.1 μg/mL mNGF as induction medium. The rats BMSCs of passage 3 were cultured in the medium with or without previous brain tissue extracts. The expressions of neuron specific encolase (NSE) were detected after 3 days by immunocytochemistry.RESULTS: There were a few NSE in the normal control group. The expression of NSE of treatment group were significantly higher than normal control group(P<0.01).CONCLUSION: mNGF can induce BMSCs into neural cells.

Key words: Mouse nervous growth factor, Bone marrow stromal cells, Cell differentiation

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