The influence of palmitic acid to TLRs and inflammatory factors of C2C12 cell line

Abstract

Abstract: AIM: To discuss the influence of palmitic acid to TLRs and inflammatory factors of C2C12 cells, and analyze the relationship between insulin resistance (IR) and immuno-inflammation induced by TLRs. METHODS: Palmitic acid in different concentration was co-cultured with C2C12 cells for 16 hours, after that, the consumption of glucose was analyzed to estimate the cell model of IR, TLR1-5 mRNA was observed with real-time PCR and the contents of TNF-α and IFN-β were measured with ELISA method. RESULTS: Palmitic acid decreased the glucose consumption of cells, showed IR character. It also obviously induced the hyper-expression of TLR2,TLR4 mRNA and increased the amount of TNF-α and IFN-β, but had no influence to that of TLR1, TLR3 and TLR5 mRNA.CONCLUSION: Palmitic acid stimulation induced the formation of IR, this may due to the hyper-expression of TLR2, TLR4 and the inflammation which was induced by TLRs and their signaling transdution pathway..

Key words: Palmitic acid, Insulin resistance, Toll-like receptor, TNF-α, IFN-β

CLC Number:

R965.2

ZHAO Bao-sheng, LIU Yang, GAO Xiao-yan, WU Rui-guang, GUI Hai-shui, ZHU Yin-di, XU Tun-hai. The influence of palmitic acid to TLRs and inflammatory factors of C2C12 cell line[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(11): 1201-1205.

References

[1] Ieclercq IA, da Silva Morais A, Schroyen B, et al. Insulin resistance in hepatocytes and sinusoidal liver cells: mechanisms and consequences[J]. J Hepatol, 2007, 47(1): 142-156.
[2] 刘铭瑶,王文飞,李德山,等.成纤维细胞生长因子(FGF)-21改善胰岛素抵抗肝细胞对葡萄的吸收和肝糖原的合成[J]. 生物化学与生物物理进展, 2009, 36(10): 1327-1333.
[3] Crandall DL, Groeling TM, Busler DE, et al.Release of PAI-1 by human preadipocytes and adipocytes independent of insulin and IGF-1[J]. Biochem Biophysl Res Commun, 2000, 279(3): 984-988
[4] 李兰芳, 郭玉, 唐国涛, 等. Apocynin改善TNF-α诱导肝细胞胰岛素抵抗的机制研究[J]. 中国临床药理学与治疗学, 2010, 15(10): 1116-1121.
[5] Feinstein R, Kanety H, Papa MZ, et al.Tumor necrosis factor-alpha suppresses insulin-induced tyrosine phosphorylation of insulin receptor and its substrates[J]. J Biol Chem, 1993, 268(35): 26055-26058.
[6] Fukuhara A, Matsuda M, Nishizawa M, et al.Visfatin: a protein secreted by visceral fat that mimics the effects of insulin[J]. Science, 2005, 307(5708): 426-430.
[7] Hotamisligil GS.Inflammation and metabolic disorders[J]. Nature, 2006, 444: 860-867.
[8] 穆颖, 季爱玲, 刘寒强, 等. 原代培养骨骼肌细胞胰岛素抵抗模型的建立[J]. 现代生物医学进展, 2008, 8(3): 433-436.
[9] 张丽, 高聆, 梁军, 等. 棕榈酸对胰岛的脂毒性及非诺贝特的保护作用[J]. 中华内分泌代谢杂志, 2005, 21(2): 155-158.
[10] 杨宁宁, 王佑民, 陈冬, 等. 棕榈酸致大鼠L6肌细胞胰岛素抵抗与JNK1活化的关系[J]. 安徽医科大学学报, 2012, 47(3): 241-244.
[11] Poggi M, BasteIica D, GuaI P, et a1. C3H/HeJ mice carrying a toll-like receptor 4 mutation are protected against the development of insulin resistance in white adipose tissue in response to a high-fat diet[J]. Diabetologia, 2007, 50(6): 1267-1276.
[12] 方飞, 吴新荣, 罗明俐, 等. 桑叶有效部位对胰岛素抵抗HepG2细胞体外糖代谢的影响[J]. 广东药学院学报, 2011, 27(6): 2-4.
[13] Schmittgen TD, Zakrajsek BA, Mills AG, et al.Quantitative reverse transcription- polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods[J]. Anal Biochem, 2000, 285(2): 194-204.
[14] 金岳龙, 贺连平, 陈金凤, 等. C-反应蛋白基因多态性与代谢综合征的关联研究[J]. 中国临床药理学与治疗学, 2011, 16(12): 1391-1396.
[15] 沈红燕, 吴晓冬. 黄芩苷对大鼠脂肪细胞胰岛素抵抗的改善作用[J]. 中国临床药理学与治疗学, 2011, 16(3): 263-269.
[16] 李婷婷, 高丽昌, 唐禄, 等. 成纤维细胞生长因子-21改善胰岛素抵抗肝细胞对葡萄糖的吸收及机制[J]. 中国药理学通报, 2012, 28(3): 366-371.
[17] 李兰芳, 郭玉, 唐国涛, 等. 吡格列酮降低胰岛素抵抗状态下的氧化应激水平[J]. 中国临床药理学与治疗学, 2010, 15(2): 140-144.
[18] 彭恭, 刘延波, 李凌海. 等. 棕榈酸的组织吸收分布及对骨骼肌胰岛素抵抗的影响[J]. 生物物理学报, 2012, 28(1): 45-52.