Synergy inhibition effect of 17-AAG in combination with cisplatin on lung adenocarcinoma A549 cells

Abstract

Abstract: AIM: The aim of our present study is to assess of synergy inhibition effect of 17-AAG combinated with cisplatin on lung adenocarcinoma A549 cells. METHODS: MTT assay was used to detect the inhibiton of the various concentrations of 17-AAG, cisplatin, or the two combination on A549 cells. The median-effect method of Chou and Talalay was used to evaluate the combination effect. RESULTS: 17-AAG or cisplatin alone inhibited the growth of A549 cells in a concentration-dependent manner. The IC₅₀ for 48 h was 69.627 μmol/L for cisplatin, and 0.455 μmol/L for 17-AAG. The effect was greater when the two agents were combined, even at the lowest dosage combination.CONCLUSION: Our results demonstrated that the combination of 17-AAG and cisplatin showed synergy inhibition effect on lung adenocarcinoma A549 cells.

Key words: 17-AAG, Cisplatin, Lung adenocarcinoma, Cell proliferation

CLC Number:

R965.2

WANG Gui-ping, LIAO Hong-ying, WEI Min, WANG Yan, ZHOU Qiong, LIN Zhu-zhen, ZHANG Yan-tao. Synergy inhibition effect of 17-AAG in combination with cisplatin on lung adenocarcinoma A549 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(11): 1240-1244.

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