Abstract: AIM: To investigate the distribution and drug resistance of pathogens causing nosocomial infections, and consequently guide rational use of antibiotics. METHODS: A retrospective study was performed to investigate the distribution and drug resistance of gram-negative bacterial isolated from our hospital during January 2012 to June 2012, and the WHONET 5.4 software was used to analyze the data based on the guideline of CLSI 2011 version. RESULTS: 2791 bacterial strains were isolated, the most common pathogens identified were Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus lactis, the detection rate were 18.99%,15.84%,11.03%,8.53%,7.45%, respectively; Klebsiella pneumonia, Pseudomonas aeruginosa, Acinetobacter baumannii have higher drug-resistant rate, in which Acinetobacter baumannii is more likely to resistant to antibiotics. CONCLUSION: In order to restrict antibiotic resistance, appropriate use of antibiotics must be strengthened.

Key words: Bacteria Pathogens, Pathogenic bacteria, Antibiotic Resistance