Abstract: AIM: To investigate the level of anti-endothelial cell antibody (AECA) and anti-β₂GP-Ⅰantibody in serum with systemic lupus erythematosus (SLE) and observe the variation of human umbilical veins endothelial cell(HUVEC) cultured in serum with SLE. METHODS: The level of AECA and anti-β₂GP-Ⅰantibody were detected by enzyme linked immunosorbent assay(ELISA) in serum with SLE and 40 healthy persons. Besides, the systemic lupus erythematosus disease activity index(SLEDAI)were collected for analyzing the relationship of the level of AECA and anti-β₂GP-Ⅰantibody and it. The HUVEC were cultured with the serum in SLE, and the influence of it were observed by methyl thiazolyl tetrazolium(MTT). RESULTS: The level of AECA and anti-β₂GP-Ⅰantibody in serum with SLE were significantly higher than that of control. The anti-β₂GP-Ⅰantibody was positive in 33 cases among 90 patients with SLE. The level of AECA and anti-β₂GP-antibody and SLE active index (SLEDAI) of 33 patients were respectively significant higher than that of 57 patients (P<0.01, P<0.05, P<0.05). There was a significantly positive correlation between the level of AECA and anti-β₂GP-Ⅰantibody(r=0.984, P<0.01).There was a positive correlation between the level of AECA and anti-β₂GP-Ⅰantibody with SLEDAI (P<0.05). The level of anti-β₂GP-Ⅰantibody was significant correlation with the index of PT (r=0.349, P<0.05). The proliferation of endothelial cells was inhibited by the serum of SLE with high level of AECA. There was no difference among three groups: the blank control group and the healthy group and the group with low AECA. The OD of group with the serum high expression of AECA was significantly higher than that of other groups (P<0.05).CONCLUSION: The level of AECA and anti-β₂GP-Ⅰantibody was correlative with the active of SLE. AECA can inhibit the proliferation of endothelial cell in vitro.

Key words: Systemic lupus erythematosus, Anti-endothelial cell antibody, Anti-β₂GP-Ⅰantibody;, Human umbilical veins endothelial cell