The protective effects of Jidesheng Sheyao pian on lung ischemia-reperfusion injury in rats

Abstract

Abstract: AIM: To investigate the protective effects and the mechanisms of Jidesheng Sheyao pian on lung ischemia-reperfusion injury of rats.METHODS: Thirty SD male rats were divided into Jidesheng Sheyao group (n=10), control group (n=10) and ischemia-reperfusion injury group (I/R, n=10). The models of lung ischemia-reperfusion injury of SD rats were established. Then, the wet/dry ratio was measured, the histopathology of the lung injury was observed by light microscope and electron microscope, the activity of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) in the lung homogenates were detected by zymography, RT-PCR the expression of MMP-2, MMP-9 mRNA were detected in rat lung tissues.RESULTS: Compared with the I/R group, the wet/dry ratio were significantly decreased (P<0.01), the degree of lung injury showed lighter, the activity of MMP-9 in lung homogenate presented inhibition (P<0.01) in the drug group and the expression of MMP-9, MMP-2 mRNA was also depressed than that of the I/R group.CONCLUSION: Jidesheng Sheyao pian can inhibit the expression and activity of MMP-9 and MMP-2, attenuate basement membrane degradation, sequentially, has protective effects on lung ischemia-reperfusion injury in rats.

Key words: Jidesheng Sheyao pian, Lung ischemia-reperfusion injury, Matrix metalloproteinase

CLC Number:

TAO Dong-ying, NI Jing-jing, REN Dian-huan, YU Ji-mian, ZHANG Xiu-juan, WANG Yong. The protective effects of Jidesheng Sheyao pian on lung ischemia-reperfusion injury in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(11): 1219-1223.

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