Anticancer effects of hyperoside on human gastric cancer cells and its mechanisms

Abstract

Abstract: AIM: To observe the effects of hyperoside on the cell proliferation, cycle and apoptosis of human gastric cancer cell line BGC-823 and to explore its possible mechanisms. METHODS: The effects of hyperoside on the proliferation of BGC-823 cells were evaluated by MTT assay. Flow cytometry was used to analyze the cell cycle and apoptosis of the cells exposed to hyperoside. The activities of caspase 3, caspase 8 and caspase 9 were examined by spectrophotometry. RESULTS: With the hyperoside incubation for 24 h or 48 h resulted in a significant inhibition of proliferation in BGC-823 cells as a IC₅₀ of 32.14 μg/mL and 22.67 μg/mL. After treatment with hyperoside,the cell cycle was suppressed obviously at G₀/G₁ phase. The apoptotic rates were significantly higher in the cells treated with hyperoside than in the control. Comparing with the control, the activities of caspase 3, caspase 8 and caspase 9 were up-regulated in hyperoside group. CONCLUSION: Hyperoside have significant anti-gastric tumor effects by induce cell cycle arrest and apoptosis of BGC-823 cells, and intracelular caspase 3, caspase 8 and caspase 9 are closely associated with the apoptosis.

Key words: hyperoside, BGC-823 cells, cell cycle, apoptosis

CLC Number:

R965.2

SUN Shi-hua, JIANG Rong-hua, ZHU Hai-yan, JIANG Jian-jun. Anticancer effects of hyperoside on human gastric cancer cells and its mechanisms[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(9): 979-983.

References 15

[1]	Siegel R, Naishadham D, Jemal A.Cancer statistics, 2013[J]. CA Cancer J Clin,2013, 63(1): 11-30.
[2]	陈万青, 张思维, 郑荣寿, 等. 中国2009 年恶性肿瘤发病和死亡分析[J]. 中国肿瘤,2013,22(1): 2-12.
[3]	Zou Y, Lu Y, Wei D.Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L in vitro[J]. J Agric Food Chem, 2004, 52(16): 5032-5039.
[4]	Song BW, Ma CG, Xu SY.Hyperoside-a new peripheral analgesic agent[J]. Asia Pacific Journal of Pharmacology, 1988, 3(1): 1-5.
[5]	Liu Z, Tao X, Zhang C, et al.Protective effects of hyperoside (quercetin-3-o-galactoside) to PC12 cells against cytotoxicity induced byhydrogen peroxide and tert-butyl hydroperoxide[J]. Biomed Pharmacother, 2005, 59(9): 481-490.
[6]	袁丽春, 刘斌, 石任兵. HPLC 法测定不同市售荷叶药材中金丝桃苷和异槲皮苷的含量[J]. 药物分析杂志, 2010,30(1): 41-44.
[7]	Li FR, Yu FX, Yao ST, et al.Hyperoside extracted from manchurian rhododendron leaf induces apoptosis in human endometrial cancer cells through a mitochondrial pathway[J]. Asian Pac J Cancer Prev, 2012, 13(8): 3653-3656.
[8]	王丽敏, 江清林, 毕士有, 等. 金丝桃苷对体外肿瘤细胞增殖的抑制作用研究[J]. 黑龙江医药科学, 2010, 33(1): 73.
[9]	宋少刚, 杨雁. 黄芪总提物对大鼠肝星状细胞增殖及产生胶原的影响[J]. 中国临床药理学与治疗学, 2001, 6(2): 111-113.
[10]	孙宇新, 孟艳, 董震, 等. 金丝桃素光照对喉癌细胞 Hep-2 的影响[J]. 中华耳鼻咽喉头颈外科杂志, 2005, 40(2): 128-128.
[11]	刘兴吉, 李玉林, 罗毅男, 等. 金丝桃素对 C6 胶质瘤细胞周期变化的研究[J]. 中国实验诊断学, 2003, 7(1): 48-50.
[12]	王丽敏, 李爽, 张明远, 等. 金丝桃苷对人结肠癌 HCT8 细胞生长周期, 细胞凋亡及对 Caspase 蛋白活性的影响[J]. 时珍国医国药, 2012, 23(12): 3010-3011.
[13]	赵保胜, 刘洋, 徐暾海. 人参皂苷Rg1 对人胃癌 BGC-823 的抑制作用研究[J]. 中国临床药理学与治疗学, 2011, 16(4): 361-365.
[14]	Kim R, Emi M, Tanabe K.Caspase-dependent and-independent cell death pathways after DNA damage (Review)[J]. Oncology Reports, 2005, 14(3): 595-599.
[15]	吴畏, 于彩平, 李卓恒, 等. 金丝桃苷对高糖, 氧化下内皮细胞凋亡及细胞色素 C, caspase 9 和 caspase 3 表达的影响[J]. 中国医院药学杂志, 2013, 33(18): 1483-1486.