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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2014, Vol. 19 ›› Issue (9): 979-983.

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Anticancer effects of hyperoside on human gastric cancer cells and its mechanisms

SUN Shi-hua1, JIANG Rong-hua1, ZHU Hai-yan2, JIANG Jian-jun3   

  1. 1 Traditional Chinese Medical Hospital of Jiangshan, Jiangshan 324100, Zhejiang, China;
    2 Quzhou People's Hospital, Quzhou 324000, Zhejiang, China;
    3 People's Hospital of Jiangshan, Jiangshan 324100, Zhejiang, China
  • Received:2014-03-07 Revised:2014-08-04 Online:2014-09-26 Published:2014-09-26

Abstract: AIM: To observe the effects of hyperoside on the cell proliferation, cycle and apoptosis of human gastric cancer cell line BGC-823 and to explore its possible mechanisms. METHODS: The effects of hyperoside on the proliferation of BGC-823 cells were evaluated by MTT assay. Flow cytometry was used to analyze the cell cycle and apoptosis of the cells exposed to hyperoside. The activities of caspase 3, caspase 8 and caspase 9 were examined by spectrophotometry. RESULTS: With the hyperoside incubation for 24 h or 48 h resulted in a significant inhibition of proliferation in BGC-823 cells as a IC50 of 32.14 μg/mL and 22.67 μg/mL. After treatment with hyperoside,the cell cycle was suppressed obviously at G0/G1 phase. The apoptotic rates were significantly higher in the cells treated with hyperoside than in the control. Comparing with the control, the activities of caspase 3, caspase 8 and caspase 9 were up-regulated in hyperoside group. CONCLUSION: Hyperoside have significant anti-gastric tumor effects by induce cell cycle arrest and apoptosis of BGC-823 cells, and intracelular caspase 3, caspase 8 and caspase 9 are closely associated with the apoptosis.

Key words: hyperoside, BGC-823 cells, cell cycle, apoptosis

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