Abstract:

AIM: To study the protective effects of total flavonoids of Chuzhou chrysanthemum (TFCC) against cerebral ischemia reperfusion injury in rats.  METHODS: 48 SD rats were randomly assigned into 6 groups of sham operation group, model control group, nimodipine group, TFCC 40, 80 and 160 mg/kg groups, with 8 rats in each group, which employed intraperitoneal administration. The ischemic model was established with line embolism to block the middle cerebral artery of rats, with 24-hours-reperfusion after 2-hours-ischemia. 24 hours after modeling, the protective effect of TFCC was determined by behavioral neurological damage scores, water content of brain tissue, cerebral infarction volume. The content of malondi-aldehyde (MDA) in brain tissue were measured.RESULTS:40, 80 and 160 mg/kg TFCC can reduce water content of brain tissue and cerebral infarction volume, and decrease the content of MDA in brain tissue (P<0.05 or P<0.01); 160 mg/kg TFCC reduced neurobehavioral score of rats (P<0.01). CONCLUSION: TFCC has a protective effect on cerebral ischemia reperfusion injury in rats, the effect may be associated with its antioxidant activity.

Key words: total flavonoids of Chuzhou chrysanthemum, cerebral ischemia reperfusion injury, infarction volume, malondi-aldehyde