Mechanism of apoptosis of mucoepidermoid carcinoma MEC-1 cells induced by ethanol extract of Lamiophlomis rotate

Abstract

Abstract:

AIM: To investigate the pro-apoptotic of ethanol extract of Lamiophlomis rotate (EELR) on human mucoepidermoid cancer cells (MEC-1). METHODS: Sulforhodamine B (SRB) colorimetric method, cell clone formation experiment, Hoechst33258/PI staining and flow cytometry instrument technique were used to determine cell apoptosis, and Western blot analysis was used to detect alterations in cell apoptosis pathway-related proteins.RESULTS:EELR dose- and time-dependently exhibited anti-proliferation. With the increase of EELR concentration, the pro-apoptotic effect on MEC-1 was increased gradually. Moreover, Western blot analysis indicated that the expression of Bcl-2 was reduced and Bax and Casepase-3 were increased with the increasing of EELR concentration. CONCLUSION: EELR can induce MEC-1 cells apoptosis, which may be associated with the down-regulation of the Bcl-2 expression and up-regulation of the Bax and Casepase-3 expressions.

Key words: ethanol extract of Lamiophlomis rotate, mucoepidermoid carcinoma MEC-1 cell lines, cell apoptosis

CLC Number:

R965.2

MA Chenghua, ZHOU Haijing, SHI Wen, WANG Zhiqiang, YANG Lan, CHEN Ping, ZHENG Xueli, MA Rui, MA Lijuan. Mechanism of apoptosis of mucoepidermoid carcinoma MEC-1 cells induced by ethanol extract of Lamiophlomis rotate[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(3): 253-259.

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