关键词: :孤独症, 常压高氧, 学习记忆, 核转录因子κB, 大鼠

Abstract: Objective To observe the changes of learning and memory ability in valproic caid (VPA) autism model rats and the expression of NF-κB positive neurons in hippocampal CA1 region after normal pressure and high oxygen intervention. Methods To animal model of autism was establishe by the methods of Schneider, the subjects of study were randomly divided into normal pressure high oxygen group and model control group, normal control group were randomly selected from only males offspring of Wistar rat were evaluatepregnancy by intraperitoneal injection of the same amount of normal saline (each group 12).The learning and memory of the autism model rats were evaluated by the Y electricity maze test after the normal pressure and high oxygen intervention.The immunohistochemistry methods and image analysis were used to examine the number of NF-κB positive neurons in hippocampal CA1 region in different groups. Results The trying times of the normal pressure high oxygen group after treatment was less than those before treatment (31.08±0.99 vs 31.67±0.89, t=2.887, P=0.015), the memory times was increased(3.02±0.67 vs 2.62±0.52, t=-2.379, P=0.036).The number of the NF-κB positive neurons of the model control group was more than that of the normal control group, with statistical differences between them(4.67±1.58 vs 3.00±1.54, t=2.639, P=0.015).The number of the NF-κB positive neurons of the normal pressure high oxygen group was less than that of the model control group, with statistical differences between them(3.17±1.27 vs 4.67±1.58, t=2.588, P=0.017). Conclusions The pathogenesis of autism may be related to the expression level of NF-κB in the hippocampal CA1 region .The intervention of normal pressure high oxygen can reduce the expression of NF-κB in the hippocampal CA1 region of VPA autism model rats and improve their learning and memory ability

Key words: autism, normal pressure high oxygen, learning and memory, nuclear transcription factor kappa B, rats