关键词: NOD样受体, 炎症反应, 抗感染免疫, 早产儿, 胎龄, 出生体重

Abstract: Objective To compare the difference of the expression of NOD-like receptors (NOD1 and NOD2), IL-6 and TNF-α in preterm infants in different gestational age and birth weight groups, in order to analyze the relationship among gestational age, birth weight and immune function in preterm infants. Methods Blood samples of preterm infants in the neonatal department of Childerns Hospital of Soochow University were collected from April 2016 to January 2017. The peripheral blood mononuclear cells of the preterm infants were collected, and were stimulated for 24 hours by Tri-DAP and MDP.The expression levels of NOD1 and NOD2 gene in the cells and IL-6, TNF-α in the supernatant were tested. Results The expression levels of NOD1 mRNA and IL-6 in preterm infants with gestational age<32 weeks were significantly lower than those with gestational age of 34-＜36 weeks (P<0.05).The expression of NOD1 mRNA in preterm infants with birth weight<1.5 kg was significantly lower than those with birth weight over 1.5 kg.And the expression of NOD2 mRNA and IL-6 was significantly lower than that with 2.0-2.5 kg (P<0.05).Moreover, the levels of NOD1 mRNA and NOD2 mRNA were positively correlated with birth weight(r=0.352,0.306,P<0.05), but were not related to gestational age.The expression of TNF-α was positively correlated with both gestational age and birth weight(r=0.380,0.289,P<0.05). Conclusions Preterm infants with lower birth weight has lower levels of NOD-like receptor and poorer immunity to infection.Birth weight has greater influence on immune function in premature infants compared with gestational age.

Key words: NOD-like receptor, inflammatory response, anti-infection immunity, premature infants, gestational age, birth weight