TLRs信号通路和炎症因子的表达与反复呼吸道感染的关系分析

doi:10.11852/zgetbjzz2020-0897

摘要/Abstract

摘要： 目的研究Toll样受体(TLRs)信号通路和炎症因子的表达与反复呼吸道感染的关系,为临床治疗提供监测靶点。方法将汕头市龙湖区第二人民医院儿科2019年1-12月门诊收治的150例反复呼吸道感染患儿纳入研究,记作病例组,另取同期于本院进行体检的健康儿童150例作为对照组。比较病变组和对照组儿童TLRs信号通路和炎症相关因子水平,并作相关性分析。此外,对比病变组和对照组儿童氧化应激反应相关指标水平。结果病例组患儿Toll样受体2(TLR2)及TLR4水平显著高于对照组,差异有统计学意义(t=69.981,101.427,P＜0.001)。病例组患儿血清白细胞介素-4(IL-4)、白细胞介素-10(IL-10)及肿瘤坏死因子-α(TNF-α)水平显著高于对照组(t=78.453,63.388,116.697,P<0.001),干扰素γ(INF-γ)显著低于对照组(t=70.489,P<0.001)。Pearson相关性分析显示:反复呼吸道感染患儿TLR2、TLR4和血清IL-4、IL-10、TNF-α水平均呈正相关(P<0.05或<0.01),与INF-γ水平呈负相关(P<0.001)。病变组超氧化物歧化酶(SOD)、丙二醛(MDA)及过氧化氢酶(CAT)水平与对照组相比,差异均有统计学意义(t=28.633,57.277,50.103,P＜0.001)。结论 TLRs信号通路和炎症因子均可能参与反复呼吸道感染的发生、发展过程,且反复呼吸道感染患者存在明显的氧化应激状态异常。

关键词: 反复呼吸道感染, Toll样受体信号通路, 炎症因子, 氧化应激反应

Abstract: Objective To study the relationship between Toll like receptor (TLRs) signaling pathway and expression of inflammatory factors and recurrent respiratory tract infection, in order to provide monitoring targets for clinical treatment. Methods A total of 150 children with recurrent respiratory tract infection treated in the Second People's Hospital of Longhu District from January to December 2019 were included in this study and were selected as case group. Meanwhile, 150 healthy children who took physical examination were selected as control group. Then the levels of TLRs signaling pathway, inflammatory related factors and oxidative stress related indexes were compared between the two groups. Results The levels of Toll like receptor 2 (TLR2) and TLR4 in the case group were significantly higher than those in the control group(t=69.981, 101.427, P＜0.001). Additionally, the levels of serum interleukin-4 (IL-4),interleukin-10(IL-10) and interferon gamma (INF-γ) in the case group were significantly higher than those in control group(t=78.453, 63.388, 116.697, P＜0.001), while the level of tumor necrosis factor-α (TNF-α) was significantly lower in the case group(t=70.489, P＜0.001). Pearson correlation analysis showed that the TLR2 and TLR4 levels were positively related to the levels of IL-4, IL-10 and TNF-α, but negatively correlated with INF-γ levels(P＜0.05). The differences on superoxide dismutase(SOD), malondialdehyde(MDA) and catalase(CAT) between the case group and control group were significant(t=28.633, 57.277, 50.103, P＜0.001). Conclusion The TLRs signaling pathway and inflammatory factors may be involved in the occurrence and development of recurrent respiratory tract infection, and children with recurrent respiratory tract infection apparently have abnormal oxidative stress.

Key words: recurrent respiratory tract infection, Toll like receptor signaling pathway, inflammatory factors, oxidative stress response

中图分类号:

R725.6

吴少辉, 黄晓冬, 徐智航. TLRs信号通路和炎症因子的表达与反复呼吸道感染的关系分析[J]. 中国儿童保健杂志, 2021, 29(2): 205-208.

WU Shao-hui, HUANG Xiao-dong, XU Zhi-hang. Analysis of the relationship between the expression of TLRs signaling pathway and inflammatory factors and recurrent respiratory tract infection[J]. journal1, 2021, 29(2): 205-208.

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