慢性间断性低氧对幼鼠肾脏超微结构和功能的影响

中国儿童保健杂志 ›› 2013, Vol. 21 ›› Issue (7): 708-710.

慢性间断性低氧对幼鼠肾脏超微结构和功能的影响

陈燕¹,侯清华²,卢超²,叶敏²,叶晓芬²,赵春玲¹

泸州医学院 1 生理学教研室;
2 临床医学系,四川泸州 646000

收稿日期:2013-02-20 发布日期:2013-07-06 出版日期:2013-07-06
通讯作者: 赵春玲,E-mail:zhchlzth@21cn.com
作者简介:陈燕(1979-),女,讲师,硕士研究生,主要研究方向为缺氧性脑损伤的发病机制和防治。
基金资助:
四川省卫生厅课题(2007-431)

Effect of chronic intermittent hypoxia on renal ultrastructure and function in immature rats.

CHEN Yan¹,HOU Qing-hua²,LU Chao²,YE Min²,YE Xiao-fen²,ZHAO Chun-ling¹.

1 Department of Physiology;
2 Department of Clinical Medicine,Luzhou Medical College,Luzhou,Sichuan 646000,China

Received:2013-02-20 Online:2013-07-06 Published:2013-07-06

摘要/Abstract

摘要： 目的探讨慢性间断性低氧(chronic intermittent hypoxia,CIH)对幼鼠肾脏超微结构和功能的影响。方法雄性21日龄SD幼鼠22只,体质量(50±5)g,随机均分为对照组和慢性间断性低氧组(CIH组)。CIH组每日间断缺氧8 h,连续缺氧6周。利用透射电镜观察肾脏超微结构变化;利用比色法测定血清丙二醛(malondialdehyde,MDA)、肾组织丙二醛水平,测定尿N-乙酰-β-D氨基葡萄糖苷酶(NAG)活性和肌酐(Cr)含量。结果透射电镜观察发现对照组肾脏超微结构基本正常,CIH组肾小球内皮细胞水肿,足细胞突起融合,肾小管上皮细胞水肿明显。与对照组比较,CIH组血清MDA、肾组织MDA水平明显升高(P<0.01)。与对照组比较,CIH组尿NAG活性明显升高(P<0.01),尿Cr含量明显降低(P<0.05)。结论 CIH可导致幼鼠肾脏超微结构改变和肾功能损害,这可能与缺氧后产生过多的氧自由基有关。

关键词: 低氧, 肾脏, 超微结构, 功能, 幼鼠

Abstract: Objective To investigate the effect of chronic intermittent hypoxia (CIH) on renal ultrastructure and function in immature rats. Methods A total of 22 postnatal day 21 (P21) Sprague-Dawley (SD) male rats were randomly averagely divided into two groups:control group and CIH group.CIH-handled rats were exposed to intermittent hypoxia in designed cabin 8 hours every day,and the duration of hypoxia was 6 weeks.The renal ultrastructure was observed by transmission electron microscopy.The value of serum malondialdehyde (MDA) and renal malondialdehyde,activity of urinary enzyme N-β-D-glucosaminidase as well as content of the urinary creatinine were detected by colorimetry. Results The renal ultrastructure of control group were normal.The glomerular endothelial cells showed edema and the foot process fusion were demonstrated in CIH group.Renal tubular epithelial cells appeared obvious edema in CIH group.Compared with the control group,the value of serum MDA and renal MDA were increased obviously (P<0.01).There were significantly increased in activity of Urinary N-acetyl-D-glucosaminidase (NAG) of CIH group (P<0.01),but the content of the urinary creatinine were less than those in control group (P<0.05). Conclusion The study demonstrated CIH exposures result in changes of renal ultrastructure and impairment of renal function,which may be associated with the produce of excessive oxygen radicals after hypoxia.

Key words: hypoxia, kidney, ultrastructure, function, immature rats

中图分类号:

Q954.5

陈燕,侯清华,卢超,叶敏,叶晓芬,赵春玲. 慢性间断性低氧对幼鼠肾脏超微结构和功能的影响[J]. 中国儿童保健杂志, 2013, 21(7): 708-710.

CHEN Yan,HOU Qing-hua,LU Chao,YE Min,YE Xiao-fen,ZHAO Chun-ling.. Effect of chronic intermittent hypoxia on renal ultrastructure and function in immature rats.[J]. journal1, 2013, 21(7): 708-710.

参考文献

[1] 周永海,蔡晓红,张存雪,等.慢性间歇低氧对幼鼠学习记忆的影响[J].中国行为医学科学,2007,16(1):13-15.
[2] 邵川,隆玄,李善群.阻塞性睡眠呼吸暂停低通气综合征肾脏损害及其机制研究进展[J].中国临床医学,2011,18(5):723-725.
[3] 杨宇,谭胜玉,张新民,等.间歇性缺氧对大鼠认知功能和海马CA1区超微结构的影响[J].中国神经免疫学和神经病学杂志,2007,14(3):157-163.
[4] Liner LH,Marcus CI.Ventilatory management of sleep-disordered breathing in children[J].Curr Opin Pediatr,2006,18(3):272-276.
[5] Chou YT,Lee PH,Yang CT,et al.Obstructive sleep apnea:a stand-alone risk factor for chronic kidney disease[J].Nephrol Dial Transplant,2011,26(7):2244-2250.
[6] Duzova A,Bakkaloglu A,Kalyoncu M,et al.Etiology and outcome of acute kidney injury in children[J].Pediatr Nephrol,2010,25(8):1453-1461.
[7] Jiang W,Duong TM,de Lanerolle NC.The neuropathology of hyperthermic seizures in the rat[J].Epilepsia,1999,40(1):5-19.
[8] 传良敏,洪华,邓君,等.尿N-乙酰-β-D-氨基葡萄糖苷酶对监测早期肾功能损害的价值[J].检验医学与临床,2009,15(6):1244-1245.
[9] 李雪竹,严海东.尿β2微球蛋白、NAG测定对肾功能评价的意义及其与年龄的关系[J].中国医科大学学报,2006,35(3):314-315.
[10] 赵卫平,张红,邱嵘,等.碳离子全身辐照对小鼠血清及肝脏中超氧化物歧化酶活性、丙二醛含量和脑组织中还原性谷胱甘肽浓度的影响[J].辐射研究与辐射工艺学报,2007,25(1):33-36.
[11] Molitoris BA,Sutton TA.Endothelial,injury and dysfunction:role in the extension phase of acute renal failure[J].Kidney Int,2004,66(2):496-499.
[13] Sutton TA,Fisher CJ,Molitoris BA.Microvascular endothelial injury and dysfunction during ischemic acute renal failure[J].Kidney Int,2002,62(5):1539-1549.

[1]	曹慧, 严双琴, 关宏岩. 亲子互动对儿童早期发展结局的影响[J]. 中国儿童保健杂志, 2023, 31(7): 770-774.
[2]	马冰洁, 卢少敏, 邢艳菲, 梁晶晶. 超重肥胖儿童智力水平及执行功能的表现特征[J]. 中国儿童保健杂志, 2023, 31(6): 601-605.
[3]	王曼, 肖莹莹, 张园园, 张立新. 数字OT系统辅助下分析全身振动训练对脑瘫患儿精细运动能力影响的临床研究[J]. 中国儿童保健杂志, 2023, 31(5): 491-496.
[4]	郑亭玉, 张春宇, 杨飞, 郑芳, 张梦霞, 夏露萍, 徐孙超. 营养支持小组管理模式对早产儿生长发育及免疫功能的影响[J]. 中国儿童保健杂志, 2023, 31(5): 555-558.
[5]	宋超, 胡莉菲, 吴玲玲, 江忠权. 孤独症谱系障碍儿童合并智力功能缺陷的GBM预测模型构建与评价[J]. 中国儿童保健杂志, 2023, 31(3): 241-245.
[6]	彭莲华, 刘冬冬, 龙楠楠, 徐萍, 陈阳, 毛平. 童年不良经历青少年执行功能现状及影响因素研究进展[J]. 中国儿童保健杂志, 2023, 31(3): 299-303.
[7]	马良, 王艳霞, 冉霓, 单延春, 刘小梅, 刘媛媛, 衣明纪. 功能性构音障碍儿童口部运动功能及其与辅音错误发音相关性[J]. 中国儿童保健杂志, 2023, 31(2): 156-161.
[8]	曹冰冰, 林多华, 杜佩珍, 颜陶, 程建婷. 多种实验室指标和炎性因子表达水平与新生儿神经发育的相关性研究[J]. 中国儿童保健杂志, 2023, 31(2): 205-209.
[9]	肖蓉, 张玉玲. 重度贫血和肾上腺皮质功能减退症为首发症状的危重型甲基丙二酸血症1例[J]. 中国儿童保健杂志, 2023, 31(2): 229-232.
[10]	彭莲华, 刘冬冬, 朱肖, 姜珊, 徐萍, 毛平. 睡眠质量和抑郁在童年不良经历青少年神经质人格与执行功能之间的链式中介作用[J]. 中国儿童保健杂志, 2022, 30(8): 851-855.
[11]	周春明, 陈土容, 陈艺, 曹建国. 脊髓性肌萎缩症的运动功能评估及临床研究进展[J]. 中国儿童保健杂志, 2022, 30(7): 741-745.
[12]	房海波, 王荣, 褚琳娜, 冯艳芳, 白容荣, 郭丰桐. 血清维生素D水平与伴中央颞区棘波的儿童良性癫痫共患注意缺陷多动障碍患儿认知功能的相关性分析[J]. 中国儿童保健杂志, 2022, 30(7): 775-778.
[13]	陆祖韬, 倪钰飞, 顾秋燕, 徐仁杰, 葛向阳. 离心收缩训练联合强制诱导运动疗法对痉挛型偏瘫儿童上肢功能及表面肌电特征的影响[J]. 中国儿童保健杂志, 2022, 30(7): 801-805.
[14]	牛玉硕, 冉霓, 胡晓雯, 李葵南, 杨秀玲. 学龄前儿童功能性便秘风险预测模型的构建[J]. 中国儿童保健杂志, 2022, 30(6): 612-616.
[15]	陈婧, 童光磊. 脑瘫儿童进食功能分级系统及营养不良分析[J]. 中国儿童保健杂志, 2022, 30(5): 544-547.