孤独症大鼠前额叶皮质突触相关蛋白的表达

doi:10.11852/zgetbjzz2017-25-05-11

中国儿童保健杂志 ›› 2017, Vol. 25 ›› Issue (5): 470-473.DOI: 10.11852/zgetbjzz2017-25-05-11

孤独症大鼠前额叶皮质突触相关蛋白的表达

陈运华¹, 勾云¹, 周波², 李宏伟¹, 刘芬³, 童雪涛³, 文敏¹

1 贵州医科大学人体解剖学教研室,贵州贵阳 550025;
2 贵州医科大学细胞生物学教研室,贵州贵阳 550025;
3 贵州医科大学附属医院儿科,贵州贵阳 550004

收稿日期:2016-10-25 发布日期:2017-05-10 出版日期:2017-05-10
通讯作者: 文敏,E-mail:670418077@qq.com
作者简介:陈运华(1985-),男,硕士研究生,主要从事神经发育障碍性疾病的研究。
基金资助:
贵州省科技厅联合基金(黔科合LH字[2015]7351); 贵州省科技厅资助项目(黔科合字[2010]3082号)

Expression of synaptic associated proteins in the prefrontal cortex of autism rats

CHEN Yun-hua¹, GOU Yun¹, ZHOU Bo², LI Hong-wei¹, LIU Fen³, TONG Xue-tao³, WEN Min¹

1 Department of Anatomy,Guizhou Medical University,Guiyang,Guizhou 550025,China;
2 Department of Biological Technology,Guizhou Medical University,Guiyang,Guizhou 550025,China;
3 Department of Pediatrics, Affiliated Hospital of Guizhou Medical University,Guiyang,Guizhou 550004,China

Received:2016-10-25 Online:2017-05-10 Published:2017-05-10
Contact: WEN Min,E-mail:670418077@qq.com

摘要/Abstract

摘要： 目的观察孤独症大鼠突触相关蛋白突触素(SYN)、突触后致密蛋白-95(PSD-95)、桥尾蛋白(gephyrin)在前额叶皮质的表达变化,探讨突触相关蛋白在孤独症发病中的作用。方法采用孕12.5 d(E12.5)一次性腹腔注射丙戊酸钠(600 mg/kg)的孕鼠所产下的子代鼠作为模型组,同样方法注射同等剂量的生理盐水的孕鼠所产下子代鼠作为正常组。通过斜板实验、睁眼实验、三箱实验验证模型是否成功;Western blotting方法对比生后42天两组大鼠突触相关蛋白SYN、PSD-95、gephyrin在前额叶皮质的表达变化。结果 1)成功建立孤独症动物模型:与正常组比较,模型组大鼠生长发育迟缓;社会交往能力异常、对新鲜事物偏好障碍,差异均有统计学意义(P均<0.05);2)Western blotting结果显示:与正常组比较,孤独症大鼠突触相关蛋白SYN、PSD-95表达显著增多(P<0.05),gephyrin蛋白表达显著减少(P<0.05)。结论孤独症大鼠前额叶皮质突触蛋白表达异常。

关键词: 孤独症, 突触素, 突触后致密蛋白-95, 桥尾蛋白, 前额叶皮质

Abstract: Objective To observe the change of the synapse associated protein synaptophysin (SYN),postsynappptic density protein-95 (PSD-95),gephyrin in the prefrontal cortex of the rat model of autism and explore the role of synapse associated protein in the pathogenesis of autism. Method sThe autism model group was obtained in the offspring female Wister rats those received a single intraperitoneal injection of sodium valproate (600mg/kg) on the 12.5th pregnancy day.Pregnant rats received the intraperitoneal injection of saline,whose offspring were defined as normal group.To confirm whether the animal model were successfully established,the model group were compared the oblique board test,opening eyes test and three-chamber sociability test with control group.Western blotting was used to detected the expressed level of SYN,PSD-95,gephyrin in prefrontal cortex of model group and control group in the 42th day after birth. Results 1)The animal model of autism was successfully established.Compared with control rats,the autistic ones were slower to grow,poorer social interaction ability,and the preference for fresh things was disorder,the differences were statistically significant(all P<0.05);2)Compared with control group,the model group significantly increased the expression level of SYN and PSD-95 (P<0.05) and decreased the expression level of gephyrin(P<0.05) in prefrontal cortex. Conclusion The expression of synaptic associated proteins in the prefrontal cortex of autism rats is abnormal.

Key words: autism, synaptophysin, postsynappptic density protein-95, gephyrin, prefrontal cortex

中图分类号:

R179

陈运华, 勾云, 周波, 李宏伟, 刘芬, 童雪涛, 文敏. 孤独症大鼠前额叶皮质突触相关蛋白的表达[J]. 中国儿童保健杂志, 2017, 25(5): 470-473.

CHEN Yun-hua, GOU Yun, ZHOU Bo, LI Hong-wei, LIU Fen, TONG Xue-tao, WEN Min. Expression of synaptic associated proteins in the prefrontal cortex of autism rats[J]. journal1, 2017, 25(5): 470-473.

参考文献

[1] Buckley AW,Holmes GL.Epilepsy and autism[J].Cold Spring Harb Perspect Med,2016,6(4).doi:10.1101/cshperapect.a022749.
[2] Lai MC,Lombardo MV,Baron-Cohen S.Autism[J].Lancet,2014,383(9920):896-910.
[3] 武丽杰.我国孤独症谱系障碍流行病学现状及趋势[J].中国实用儿科杂志,2013,28(8):571-576.
[4] Curatolo P,Ben-Ari Y,Bozzi Y,et al.Synapses as therapeutic targets for autism spectrum disorders:an international symposium held in pavia on july 4th,2014[J].Front Cell Neurosci,2014,8:309.
[5] 邵世怡,陈琳,蒋权,等.基于突触功能异常的自闭症发病机制研究进展[J].中国现代应用药学,2016,33(4):501-508.
[6] Schneider T,Przewlocki R.Behavioral alterations in rats prenatally exposed to valproic acid:animal model of autism[J].Neuropsychopharmacology,2005,30(1):80-89.
[7] Abrahams BS,Geschwind DH.Advances in autism genetics:on the threshold of a new neurobiology[J].Nat Rev Genet,2008,9(5):341-355.
[8] Rasalam AD,Hailey H,Williams JH,et al.Characteristics of fetal anticonvulsant syndrome associated autistic disorder[J].Dev Med Child Neurol,2005,47(8):551-555.
[9] Stromland K,Nordin V,Miller M,et al.Autism in thalidomide embryopathy:a population study[J].Dev Med Child Neurol,1994,36(4):351-356.
[10] Kim KC,Kim P,Go HS,et al.The critical period of valproate exposure to induce autistic symptoms in Sprague-Dawley rats[J].Toxicol Lett,2011,201(2):137-142.
[11] Rodier PM,Ingram JL,Tisdale B,et al.Linking etiologies in humans and animal models:studies of autism[J].Reprod Toxicol,1997,11(2-3):417-422.
[12] Yang EJ,Ahn S,Lee K,et al.Early Behavioral abnormalities and perinatal alterations of PTEN/AKT pathway in valproic acid autism model mice[J].PLoS One,2016,11(4):e153298.
[13] Cheaha D,Bumrungsri S,Chatpun S,et al.Characterization of in utero valproic acid mouse model of autism by local field potential in the hippocampus and the olfactory bulb[J].Neurosci Res,2015,98:28-34.
[14] 文敏,赵晶,鲍星星,等.孤独症大鼠前额叶皮质神经元凋亡相关蛋白的表达[J].山东大学学报:医学版,2014,52(2):49-51.
[15] Gatto C L,Broadie K.Genetic controls balancing excitatory and inhibitory synaptogenesis in neurodevelopmental disorder models[J].Front Synaptic Neurosci,2010,2:4.
[16] 刘轲,鲁红伟,冯静静,等.脑脉通联合骨髓间充质干细胞移植对脑缺血再灌注大鼠神经细胞中SYN-38和PSD-95表达变化的影响[J].时珍国医国药,2015,26(5):1048-1051.
[17] Alvarez VA,Sabatini BL.Anatomical and physiological plasticity of dendritic spines[J].Annu Rev Neurosci,2007,30(1):79-97.
[18] Courchesne E,Mouton PR,Calhoun ME,et al.Neuron number and size in prefrontal cortex of children with autism[J].JAMA,2011,306(18):2001-2010.
[19] 刘夫军,袁向山,刘静,等.小胶质细胞在VPA孤独症模型大鼠脑增龄过程中的变化[J].神经解剖学杂志,2015,31(3):275-281.
[20] Xu W.PSD-95-like membrane associated guanylate kinases (PSD-MAGUKs) and synaptic plasticity[J].Curr Opin Neurobiol,2011,21(2):306-312.
[21] Petrini EM,Ravasenga T,Hausrat TJ,et al.Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP[J].Nat Commun,2014,5:3921.
[22] Choii G,Ko J.Gephyrin:a central GABAergic synapse organizer[J].Exp Mol Med,2015,47(2):e158.

孤独症大鼠前额叶皮质突触相关蛋白的表达

Expression of synaptic associated proteins in the prefrontal cortex of autism rats

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