EV71感染手足口病严重程度与IL-6、IL-1β基因多态性的相关性研究

doi:10.11852/zgetbjzz2018-26-11-18

中国儿童保健杂志 ›› 2018, Vol. 26 ›› Issue (11): 1228-1232.DOI: 10.11852/zgetbjzz2018-26-11-18

EV71感染手足口病严重程度与IL-6、IL-1β基因多态性的相关性研究

李梅¹,李亚萍¹,邓慧玲^1,3,张雯²,马乐¹,王立群¹,吴媛¹

1 西安交通大学第二附属医院感染科,陕西西安 710004;
2 西安交通大学第二附属医院检验科,陕西西安 710004;
3 西安市儿童医院感染科,陕西西安 710043

收稿日期:2018-05-07 修回日期:2018-06-11
通讯作者: 李亚萍,E-mail:liyaping8605@126.com
作者简介:李梅(1986-),女,陕西人,主治医师,硕士学位,主要研究方向为感染性疾病。
基金资助:
国家自然科学基金(81701632);陕西省社会发展攻关项目(2017SF-154)

Research on the association of interleukin-6 and interleukin-1β gene polymorphism with severity of hand foot and mouth disease induced by enterovirus 71

LI Mei¹, LI Ya-ping¹, DENG Hui-ling^1,3, ZHANG Wen², MA Le¹, WANG Li-qun¹, WU Yuan¹

1 Department of Infectious Diseases, the Second Affiliated Hospital of Medical College,Xi′an Jiaotong University, Xi′an,Shaanxi 710004,China;
2 Department of Clinical Laboratory, the Second Affiliated Hospital of Medical College, Xi′an Jiaotong University, Xi′an, Shaanxi 710004,China;
3 Department of Infectious Diseases, Xi′an Children′s Hospital, Xi′an 710043, China

Received:2018-05-07 Revised:2018-06-11
Contact: LI Ya-ping, E-mail:liyaping8605@126.com

摘要/Abstract

摘要： 目的探讨炎性细胞因子白介素-6(IL-6)、IL-6R、IL-1β、IL-1RN基因多态性与肠道病毒71型(EV71)感染手足口病(HFMD)的严重程度的相关性,为重症HFMD的早期发现和患病风险的评估提供依据。方法以西安市儿童医院和西安交通大学第二附属医院感染科收住的180例EV71 HFMD患儿为研究对象,其中重症组108例,普通组72例。采用SNPscan^TM多重SNP技术检测上述样本IL-6 rs1800796、rs2069837,IL-6R rs2228145、rs4845617,IL-1β rs16944、rs1143627,IL-1RN rs315952、rs9005位点的基因分型。结果 180例EV71感染患儿中,IL-6 rs1800796 G等位基因的重症化风险升高(OR=1.312,95%CI:1.041~1.653),IL6-R rs2228145 C等位基因重症化风险升高(OR=1.330,95%CI:1.064~1.663),而IL-1β rs1143627 G等位基因是保护因素(OR=0.790,95%CI:0.635~0.981)。IL-6 rs2069837、IL-6R rs4845617,IL-1β rs16944、IL-1RN rs315952、rs9005的基因多态性与疾病严重程度无明显相关性。结论 IL-6 rs1800796、IL-6R rs2228145、IL-1β rs1143627基因多态性与EV71 HFMD患儿严重程度相关联。

关键词: 肠道病毒71型, 白介素-6, 白介素-1β, 基因多态性, 重症化

Abstract: Objective To investigate the association of inflammatory cytokines including interleukin-6 (IL-6), IL-6R, IL-1β and IL-1RN gene polymorphism with severity of hand foot and mouth disease (HFMD) induced by enterovirus 71 (EV71), in order to provide evidence for early recognition and assessment of severe HFMD.Methods A total of 180 EV71 HFMD cases including 108 severe cases and 72 mild cases from the Second Affiliated Hospital of Xi′an Jiaotong University and Xi′an Children′s Hospital were randomly enrolled in this study. All participants were genotyped for the most common IL-6, IL-6R, IL-1β and IL-1RN single-nucleotide polymorphisms (SNPs) using the SNPscan multiple SNP typing method. The gene type of rs1800796, rs2069837 of IL-6, rs2228145, rs4845617 of IL-6R,rs16944, rs1143627 of IL-1β and rs315952, rs9005 of IL-1R among 180 cases were detected by SNPscan kit.Results The severity risk was significantly increased in IL-6 rs1800796 allele G(OR=1.312, 95% CI:1.041~1.653)and IL6-R rs2228145 allele C (OR=1.330, 95% CI:1.064～1.663), while it was reduced in IL-1β rs1143627 allele G (OR=0.790,95% CI:0.635~0.981). No association of gene polymorphism with severity of EV71 HFMD was observed in IL-6 rs2069837, IL-6R rs4845617, IL-1β rs16944, IL-1RN rs315952 and IL-1RN rs9005.Conclusion The gene polymorphism of IL-6 rs1800796, IL-6R rs2228145 and IL-1β rs1143627 were associated with severity of EV71 HFMD.

Key words: enterovirus 71, interleukin-6, interleukin-1β, polymorphism, severity

中图分类号:

R179

李梅,李亚萍,邓慧玲,张雯,马乐,王立群,吴媛. EV71感染手足口病严重程度与IL-6、IL-1β基因多态性的相关性研究[J]. 中国儿童保健杂志, 2018, 26(11): 1228-1232.

LI Mei, LI Ya-ping, DENG Hui-ling, ZHANG Wen, MA Le, WANG Li-qun, WU Yuan. Research on the association of interleukin-6 and interleukin-1β gene polymorphism with severity of hand foot and mouth disease induced by enterovirus 71[J]. journal1, 2018, 26(11): 1228-1232.

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