Role of L-thyroxine on brain tissue hypoxia-inducible factor 1α expression after hypoxia-ischemia brain damage in neonatal rats.

doi:10.11852/zgetbjzz2014-22-07-11

Abstract

Abstract: Objective To observe the expression of hypoxia-inducible factor 1α(HIF-1α) in rat brain after hypoxia-ischemia brain damage (HIBD) and L-thyroxine (L-T4) of different dose treated. Methods Eighty postnatal 7 days old Sprague-Dawley(SD) rats were randomly divided into 5 groups :the sham operation group, hypoxia-ischemia group (HI), menstruum-treated group, L-T4-treated groups of low and high dose.The rats of menstruum-treated, L-T4-treated groups of low and high dose were respectively daily administrated of intraperitoneal injection of menstruum of the equal volume and 2 μg/100 g L-T4, 3.5 μg/100 g, once a day, for 5 days.The expression of HIF-1α were detected by immunohistochemistry.Reverse transcription-polymerase chain reaction(RT-PCR) was used to detect the level of HIF-1α mRNA. Results Compared with the sham operation group, the levels of HIF-1α protein(38.581±2.846) and HIF-1α mRNA(0.174±0.015) were upregulated in HI group(72.795±6.121, 0.448±0.035 respectively), as well as in menstruum-treated group.The levels of HIF-1α protein (117.350±9.374, 142.842±8.948 respectively)and HIF-1α mRNA(0.618±0.042, 0.711±0.049 respectively) in L-T4-treated groups of low and high dose were higher than HI group(P<0.05).The influence of L-T4 on HIF-1α protein and HIF-1α mRNA was dose dependent, namely with the increase of dose, their expression increased (P<0.05). Conclusion L-T4 upregulats HIF-1α mRNA and protein expression in neonatal rats with HIBD.

Key words: hypoxia-inducible factor 1α, L-thyroxine, hypoxia-ischemia brain damage, neonatal rat

摘要： 目的探讨不同剂量左甲状腺素(L-T4)对缺氧缺血性脑损伤(hypoxia-ischemia brain damage, HIBD)新生大鼠脑组织缺氧诱导因子-1α(hypoxia-inducible factor 1α, HIF-1α)的调节作用。方法 7日龄SD大鼠随机分为假手术组、缺氧缺血组(HI)、溶剂组及L-T4低、高剂量组。低、高剂量L-T4干预组及溶剂对照组分别于HIBD后当日起腹腔注射2 μg/100 g L-T4、3.5 μg/100 g L-T4及等体积溶剂, 每日1次, 共5 d。应用免疫组化法检测鼠脑HIF-1α蛋白, RT-PCR法检测HIF-1αmRNA。结果缺氧缺血组缺血侧大脑皮质HIF-1α蛋白(72.795±6.121)及HIF-1αmRNA(0.448±0.035)表达较假手术组(依次为38.581±2.846, 0.174±0.015)增高, 差异有统计学意义(P＜0.05);L-T4低、高剂量干预组缺血侧大脑皮质HIF-1α蛋白(依次为117.350±9.374, 142.842±8.948)及HIF-1αmRNA(依次为0.618±0.042, 0.711±0.049)表达较缺氧缺血组增高, 差异有统计学意义(P＜0.05), 且L-T4对HIF-1α的影响具有剂量依赖性, 即随剂量增加, HIF-1α蛋白及HIF-1αmRNA表达增加, 两组间差异具有统计学意义(P<0.05)。结论在新生大鼠HIBD时, L-T4可上调HIF-1α蛋白及其mRNA表达, 从而对HIBD起保护作用, 且具有剂量依赖性。

关键词: 缺氧诱导因子1-α, 左甲状腺素, 缺氧缺血性脑损伤, 新生大鼠

CLC Number:

Q95-33

ZHANG Xiao-li, JIA Tian-ming, DU Kai-xian, ZHAO Shan-shan, NIU Guo-hui. Role of L-thyroxine on brain tissue hypoxia-inducible factor 1α expression after hypoxia-ischemia brain damage in neonatal rats.[J]. journal1, 2014, 22(7): 702-704.

张晓莉, 贾天明, 杜开先, 赵闪闪, 牛国辉. 不同剂量左甲状腺素对缺氧缺血性脑损伤新生大鼠脑组织HIF-1α表达的调节[J]. 中国儿童保健杂志, 2014, 22(7): 702-704.

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