Abstract: Objective To study the dose of bifidobacteria in preventing neonatal necrotizing enterocolitis (NEC) in newborn rats,and to provide theoretical for clinical rational drug use. Methods A total of 40 SD rats were divided into 5 groups randomly:A,the normal group;B,NEC model group,give them lavage with normal saline daily while making model;C,NEC model group,establish the model and give 0.4 mg/g of duplex butyric acid bacteria living bacterium(Its main effective components is bifidobacterium)to fill the stomach daily;D,NEC model group,established the model and give 4 mg/g of duplex butyric acid bacteria living bacterium to fill the stomach daily;E,NEC model group,established the model and give 20 mg/g duplex butyric acid bacteria living bacterium to fill the stomach daily;Weighed their body weights and recorded everyday.Three days later,to fast one day.Put all the newborn mice to death.Anatomy all the rats and detect the contents of TNF-α and contrast them,observe the changes of their intestinal tissue and compared them;Observe the pathological changes of the intestinal tissue with HE staining. Results The average changes of body weight were A>D>E>C>B.There was no significance between group D and group E and no significance between group B and group C.Statistical significance existed among other groups.The mean scores of their HE staining pathology were B>D>C>E>A,there was statistical significance between A and other groups only.The averages of their TNF-α were group B>group C>group D>group E>group A.Based on the analysis of the above data,there was no significance between group D and group E and no significance between group B and group C.Statistical significance existed among other groups. Conclusion The optimal dose of duplex butyric acid bacteria living bacterium in neonatal necrotizing enterocolitis mice model may be 4 to 20 mg/g.The equivalent dose of bifidobacterium is 4×10³ CFU to 20×10³CFU.

Key words: neonatal necrotizing enterocolitis, bifidobacterium, dose, newborn rats

摘要： 目的 研究双歧杆菌在预防新生鼠坏死性小肠结肠炎中应用剂量的问题,为临床合理用药提供理论依据。方法 选取新生SD大鼠40只,随机分为5组:A,正常对照组;B,NEC模型组;C,NEC模型组,每日给予0.4 mg/g的酪酸梭菌二联活菌散(主要有效成分为双歧杆菌)灌胃;D,NEC模型组,每日给予4 mg/g酪酸梭菌二联活菌散灌胃;E,NEC模型组,每日给予20 mg/g酪酸梭菌二联活菌散灌胃。每日记录体重;3 d后禁食1 d,处死所有新生鼠,取血检测TNF-α并对比;HE染色法观察肠道组织。结果 体重变化均值为A>D>E>C>B,除B与C间,D与E间差异无统计学意义,其余组两两对比差异均有统计学意义。HE染色评分均值为B>D>C>E>A,仅A与其他各组间差异有统计学意义。TNF-α均值为B>C>D>E>A,除B与C间,D与E间差异无统计学意义,其余组间均有统计学意义。综合分析,D、E的炎症程度低于B、C。结论 酪酸梭菌二联活菌散在新生鼠NEC中应用的最佳预防剂量可能为4~20 mg/g,相当于双歧杆菌4.0×10³~20×10³CFU。

关键词: 坏死性小肠结肠炎, 双歧杆菌, 剂量, 新生鼠