Abstract: Objective To analyze the expression changes of placental growth factor(PLGF) in neonatal rats with hypoxic-ischemic brain damage,and to discuss the possible roles of PLGF in alleviating cerebral ischemia,promoting regeneration of blood vessels as well as the protective effects of Shenfu injection on the expression of PLGF. Method All the neonatal rats were randomly divided into three groups:sham operation group (S),Shenfu injection treatment group (SF),and saline control group (C).They were further divided into five subgroups(n=8 per subgroup) according to the time points:6 h,12 h,1 d,3 d,7 d.Models of postnatal 7-day Sprague Dawley rats with HIBD were established with Rice methods.Group SF was treated with Shenfu injection (10 mg/kg,ip) immediately after hypoxic-ischemic brain damage (HIBD).The injection lasted for three days once a day at the same time.Group C was treated with saline in the same way with the same dosage.There was no treatment with any drugs in Group S.RT-PCR and immunohistochemistry method were used to detect the changes of PLGF in the lesion side cerebral cortex in neonatal rats. Results Expression of PLGF in group S was the same at different time points(P>0.05); In group C,the expression of PLGF started to increase at 6 h,and reached a peak at 1 d and then gradually declined.The expression level was higher than S group from 12 h to 7 d(P<0.05);In SF group,the expression increased before 6 h,and the tendency of expression was similar to C group;However it had a more obvious decline at 3 d.The whole level was apparently higher than S and C groups. Conclusion The expression levels of PLGF increase highly after hypoxic-ischemic brain damage,and Shenfu injection can protect the brain from damage effectively through facilitating the expression of PLGF.

Key words: placental growth factor, neonatal rats, hypoxic-ischemic brain damage, Shenfu injection

摘要： 目的 通过分析胎盘生长因子(PLGF)在缺氧缺血性新生大鼠病侧大脑表达,探讨其改善脑缺血、促进脑内血管再生以及参附注射液对新生大鼠缺氧缺血性脑损伤(HIBD)的保护作用。方法 新生7 d SD大鼠随机分为三组:假手术组(S)、生理盐水对照组(C)、参附治疗组(SF)。 按观察时间可依次分为6 h、12 h、1 d、3 d、7 d五个亚组,每个亚组8只。用Rice法制备新生大鼠HIBD模型。SF组在缺氧缺血性脑损伤后即刻以腹腔注射注射,其剂量为10 ml/kg,每日1次,连续3日同一时间点用药。C组则按照相同的注射方式及注射剂量,给予生理盐水。S组未给予药物治疗。其后以RT-PCR和免疫组织化学的方法检测大鼠右侧大脑皮层PLGF的表达变化。结果 S组各个时间点PLGF均有表达,无明显差异(P>0.05);C组在6 h时PLGF表达量开始增加,于1 d时达到高峰期,明显高于S组(P<0.05);SF组在6 h前PLGF表达量开始增加,整体表达趋势类似于C组,3 d时下降趋势更明显,但各时间表达量明显高于S组及C组(P<0.05)。结论 缺氧缺血性脑损伤后,PLGF表达量明显增加,参附注射液能促进PLGF大量表达,对缺氧缺血性脑损伤后的新生大鼠起到保护作用。

关键词: 胎盘生长因子, 新生大鼠, 缺氧缺血性脑损伤, 参附注射液