Changes and significance of MUC1 and IL-11 expression in neonatal necrotizing enterocolitis tissues

doi:10.11852/zgetbjzz2020-0632

Abstract

Abstract: Objective To investigate the changes and significance of type 1 mucin (MUC1) and interleukin-11 (IL-11) in the intestinal mucosa of neonatal necrotizing enterocolitis(NEC), so as to provide evidence for the prevention and treatment of NEC in clinical practice. Methods From February 2015 to July 2019, a total of 65 cases of intestinal mucosal tissue samples of postoperative NEC children in Children′s Hospital of Zhengzhou University were collected and selected as NEC group, and 30 cases of colonic specimens obtained from surgical treatment of congenital megacolon during the same period were selected as the control group.Specimens of two groups were detected by immunohistochemical staining.The positive expression rates of MUC1 and IL-11 protein were analyzed and compared between the two groups. Results Immunohistochemical staining analysis indicated that the positive expression rates of MUC1 and IL-11 protein in colonic mucosa of children in NEC group were 47.69% and 53.85%,respectively, which were 80.00% and 83.33% in control group, and the difference was significant(χ² =8.789, 7.670, P＜0.05).The positive expression rate of MUC1 and IL-11 protein in colonic mucosa were 33.33% and 40.00%, respectively, which were 60.00% and 65.71% in control group, and the difference was significant(χ² =4.605, 4.298, P＜0.05). Conclusions The expression of MUC1 and IL-11 protein in the intestinal mucosa of NEC children are significantly down-regulated, and the expression is more significantly down-regulated in the tissues with higher disease stages.Therefore, it is speculated that the decreased expression of these two proteins may be involved in the development of NEC.

Key words: Mucin type 1, interleukin-11, newborn, necrotizing enterocolitis

摘要： 目的分析1型黏蛋白(MUC1)、白细胞介素-11(IL-11)在新生儿坏死性小肠结肠炎(NEC)肠黏膜组织中的变化及其意义,以期为NEC的防治提供依据。方法选取2015年2月-2019年7月郑州大学附属儿童医院病理科收集的手术后NEC患儿回肠末端肠黏膜组织65例(NEC组)、同期因先天性巨结肠实施手术治疗获取的小肠标本30例作为对照组;采用免疫组化染色检测两组标本中MUC1、IL-11蛋白的阳性表达率,并分析不同NEC病变分期组织中MUC1、IL-11蛋白阳性表达率差异。结果经免疫组化染色分析,NEC组患儿结肠黏膜组织中MUC1蛋白阳性表达率47.69%、IL-11蛋白阳性表达率53.85%,均低于对照组(80.00%、83.33%),差异有统计学意义(χ² =8.789、7.670, P＜0.05);疾病分期为Ⅲ期的NEC组患儿结肠黏膜组织中MUC1蛋白阳性表达率33.33%、IL-11蛋白阳性表达率40.00%,均低于Ⅱ期患儿(60.00%、65.71%),差异均有统计学意义(χ² =4.605、4.298, P＜0.05)。结论 NEC患儿肠黏膜组织中MUC1蛋白、IL-11蛋白表达强度均明显下调,在疾病分期越高的组织中下调表达越明显,因此推测这两种蛋白的降低表达可能参与了疾病的发生发展。

关键词: 1型黏蛋白, 白细胞介素-11, 新生儿, 坏死性小肠结肠炎

CLC Number:

R722.1

BAI Qiong-dan, XU Bang-li, CHEN Xin-ya, YU Zeng-yuan. Changes and significance of MUC1 and IL-11 expression in neonatal necrotizing enterocolitis tissues[J]. journal1, 2020, 28(12): 1378-1381.

白琼丹, 许邦礼, 陈新亚, 余增渊. 1型黏蛋白、白细胞介素11在新生儿坏死性小肠结肠炎黏膜组织中表达的变化及其意义[J]. 中国儿童保健杂志, 2020, 28(12): 1378-1381.

References

[1] 郝武娟, 屠文娟.坏死性小肠结肠炎新生儿肠道菌群变化研究进展[J].中华实用儿科临床杂志, 2018, 33(17):1358.
[2] 唐书庆, 朱丽, 张蓉, 等.新生儿坏死性小肠结肠炎623例临床特点分析[J].中华实用儿科临床杂志, 2019, 34(15):1171-1175.
[3] 管考平, 刘树立, 叶茂, 等.经脐单切口腹腔镜手术治疗新生儿坏死性小肠结肠炎后结肠狭窄[J].中华实用儿科临床杂志, 2019, 34(8):629-630.
[4] 陈仁慧.新生儿坏死性小肠结肠炎早期诊断生物标记物的研究进展[J].中国小儿急救医学, 2018, 25(4):301-305.
[5] Brown CT, Xiong W, Olm M, et al.Hospitalized premature infants are colonized by related bacterial strains with distinct proteomic profiles[J].Mbio, 2018, 9(2):e00441-18.
[6] Akhtar K, Zayac A, Lemke S.Intestinal pneumatosis: a manifestation of rarely reported axitinib-associated necrotizing enterocolitis[J].Am J Ther, 2018, 25(6):1.
[7] 王静.新生儿坏死性小肠结肠炎发病机制研究进展[J].安徽医药, 2019, 23(6):1074-1077.
[8] Isabela Gusson Galdino dos Santos, Maria Aparecida Mezzacappa, Beatriz Regina Alvares.Radiological findings associated with the death of newborns with necrotizing enterocolitis[J].Radiol Bras, 2018, 51(3):166-171.
[9] Cerezo VN, Muñoz MR, Encinas JL, et al.Perinatal factors for necrotizing enterocolitis (NEC).A case-control study[J].Cir Pediatr, 2018, 31(2):90-93.
[10] Juhl SM, Gregersen R, Lange T, et al.Incidence and risk of necrotizing enterocolitis in Denmark from 1994-2014[J].PLoS ONE, 2019, 14(7):e0219268.
[11] Patel AL, Kim JH .Human milk and necrotizing enterocolitis[J].Semin Pediatr Surg, 2018, 27(1):34.
[12] Sato Y, Kujirai D, Emoto K, et al.Necrotizing enterocolitis associated with Clostridium butyricum in a Japanese man[J].Acute Medicine & Surgery, 2018, 5(2):194-198.
[13] Faingold R. Technical aspects of abdominal ultrasound and color Doppler assessment of bowel viability in necrotizing enterocolitis[J].Pediatr Radiol, 2018, 48(5):1-3.
[14] Patel RM, Underwood MA .Probiotics and necrotizing enterocolitis[J].Semin Pediatr Surg, 2018, 27(1):39-46.
[15] 陆田田.新生儿坏死性小肠结肠炎致病高危因素及预后状况分析[J].医学临床研究, 2018, 35(9):1826-1828.