Effects of lithium chloride on the activity of PI3K/Akt signaling pathway in hypoxic neural stem cells

doi:10.11852/zgetbjzz2017-25-02-08

journal1 ›› 2017, Vol. 25 ›› Issue (2): 135-138.DOI: 10.11852/zgetbjzz2017-25-02-08

Previous Articles Next Articles

Effects of lithium chloride on the activity of PI3K/Akt signaling pathway in hypoxic neural stem cells

CHEN Chang-chun^1,2,ZHOU Qin²,YAN Yan^1,3,WANG Qi^1,4,ZHANG Xiao-yuan^1,5,YIN Xiao-juan¹

1 Department of Neonatology,Bayi Children's Hospital Affiliated to PLA army General Hospital,Beijing 100700,China;
2 Wuxi Maternal and Child Health Care Hospital,Wuxi,Jiangsu 214002,China;
3 Linfen Maternal and Child Health Care Hospital,Linfen,Shanxi 041000,China;
4 Civil Aviation General Hospital,Beijing 100025,China;
5 Zhengzhou Children's Hospital,Zhengzhou,Henan 450053,China

Received:2016-07-22 Online:2017-02-10 Published:2017-02-10
Contact: YIN Xiao-juan,E-mail:yyinxiaojuan@163.com

氯化锂对缺氧后神经干细胞PI3K/Akt信号通道活性的影响

陈长春^1,2,周勤²,闫燕^1,3,王琪^1,4,张晓媛^1,5,尹晓娟¹

1 陆军总医院附属八一儿童医院新生儿科,北京 100700;
2 无锡市妇幼保健院,江苏无锡 214002;
3 临汾市妇幼保健院,山西临汾 041000;
4 民航总医院,北京 100025;
5 郑州市儿童医院,河南郑州 450053

通讯作者: 尹晓娟,E-mailyyinxiaojuan@163.com
作者简介:陈长春(1984-),男,江苏人,住院医师,硕士学位,主要研究方向为新生儿神经发育及修复。
基金资助:
中国博士后科学基金(20070410505)

Abstract

Abstract: Objective To investigate the effect of lithium chloride on PI3K/Akt signaling pathway in hypoxic neural stem cells (NSCs). Methods The models of hypoxic NSCs were established by culturing and identify NSCs isolated from neonatal SD rat.The NSCs of normal control group was cultured in serum-free medium.The culture medium of normal saline intervention group was immediately added normal saline,and lithium chloride intervention group was immediately added different concentration of lithium chloride.Akt /P-Akt signaling pathway markers of NSCs were detected by immunohistochemistry. Results The amount of NSCs decreased and the characteristics of the NSCs was abnormal in shape,such as low refraction,swollen perikarya or even membranolysis.The number of dead cells of NSCs in hypoxic group increased significantly (P<0.05).The activeness of NSCs in hypoxic group decreased significantly (P<0.05).Compared with hypoxic group,normal saline intervention group and 5 mM lithium chloride intervention group,P-Akt expression level of hypoxic NSCs in 1 mM lithium chloride intervention group increased significantly,while decreased significantly compared with 3 mM lithium chloride intervention group.Compared with 1 mM and 5 mM lithium chloride intervention group,P-Akt expression level of hypoxic NSCs in 3 mM lithium chloride intervention group increased significantly.Compared with other groups,the number of hypoxic NSCs positive expressing P-Akt in 3 mM lithium chloride group increased significantly (P<0.05). Conclusions Lithium chloride activates PI3K/Akt signaling pathway in hypoxic NSCs.

Key words: neural stem cells, hypoxia model, signaling pathway, lithium chloride

摘要： 目的探讨氯化锂对缺氧后神经干细胞(NSCs)磷脂酰肌醇-3-羟激酶/蛋白激酶B(PI3K/Akt)信号通道活性的影响。方法分离培养新生SD乳鼠(SD)NSCs,建立缺氧NSCs模型。正常对照组NSCs加无血清培养基,单纯缺氧组仅缺氧,生理盐水干预组缺氧后加生理盐水,三组氯化锂干预组分别加入氯化锂至所需的浓度。免疫组织化学技术检测各组Akt /P-Akt阳性细胞表达。结果缺氧NSCs形态不规则,胞体肿胀,甚至出现细胞膜破裂,数量减少,折光性减弱;缺氧NSCs死亡数较未缺氧NSCs明显增多、活性明显降低(P均<0.05)。1 mM氯化锂干预组P-Akt表达较单纯缺氧组、生理盐水干预组及5 mM氯化锂干预组强,较3 mM氯化锂干预组弱。3 mM 氯化锂干预组P-Akt表达较1 mM 、5 mM氯化锂干预组强。3 mM氯化锂干预组P-Akt阳性细胞数较单纯缺氧组、生理盐水干预组、1 mM、5 mM氯化锂干预组明显增多(P<0.05)。结论氯化锂激活缺氧后NSCs PI3K/Akt信号通路。

关键词: 神经干细胞, 缺氧模型, 信号通路, 氯化锂

CLC Number:

R179

CHEN Chang-chun,ZHOU Qin,YAN Yan,WANG Qi,ZHANG Xiao-yuan,YIN Xiao-juan. Effects of lithium chloride on the activity of PI3K/Akt signaling pathway in hypoxic neural stem cells[J]. journal1, 2017, 25(2): 135-138.

陈长春,周勤,闫燕,王琪,张晓媛,尹晓娟. 氯化锂对缺氧后神经干细胞PI3K/Akt信号通道活性的影响[J]. 中国儿童保健杂志, 2017, 25(2): 135-138.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu41.magtech.com.cn/Jwk_zgetbjzz/EN/10.11852/zgetbjzz2017-25-02-08

https://manu41.magtech.com.cn/Jwk_zgetbjzz/EN/Y2017/V25/I2/135

References

[1] Yannan CHAI,Xiaojuan YIN.Neural dysfunction and neural regeneration,a new window into the neonatal hypoxic-ischemic brain damage[J].Acta Medica Mediterranea,2014,30(1):167-173.
[2] Bang OY.Clinical trials of adult stem cell therapy in patients with ischemic stroke[J].J Clin Neurol,2016,12(1):14-20.
[3] Hong CJ,Park H,Yu SW.Autophagy for the quality control of adult hippocampal neural stem cells[J].Brain Res,2016,1649:166-172.
[4] Wang L,Jiang F,Li Q,etal.Mild hypothermia combined with neural stem cell transplantation for hypoxic-ischemic encephalopathy:neuroprotective effects of combined therapy[J].Neural Regen Res,2014,9(19):1745-1752.
[5] Kara N,Narayanan S,Belmaker RH,et al.Chronic lithium treatment enhances the number of quiescent neural progenitors but not the number of DCX-Positive immature neurons[J].Int J Neuropsychopharmacol,2015,18(7):pyv003.
[6] 陈长春,尹晓娟.锂对缺氧缺血性脑损伤神经修复机制研究进展[J].中国儿童保健杂志,2016,24(2):156-159.
[7] 王钰,尹晓娟.锂对缺氧状态下新生鼠神经干细胞损伤的影响[J].中国儿童保健杂志,2013,21(10):1045-1048.
[8] 王钰,尹晓娟.神经干细胞在治疗缺氧缺血性脑损伤中的研究进展[J].中国儿童保健杂志,2012,20(9):826-828.
[9] Chan CB,Ye K.Phosphoinositide 3-kinase enhancer (PIKE) in the brain:is it simply a phosphoinositide 3-kinase/Akt enhancer?[J].Rev Neurosci,2012,23(2):153-161.
[10] Cai M,Zhou Y,Zhou B,et al.Hypoxic conditioned medium from rat cerebral cortical cells enhances the proliferation and differentiation of neural stem cells mainly through PI3-K/Akt pathways[J].PLoS One,2014,9(11):e111938.
[11] 王丽敏,韩小唯,王超,等.钙敏感受体表达和Pi3k/Akt通路调控癫痫大鼠心肌细胞凋亡的研究[J].中国儿童保健杂志,2016,24 (5):479-482.
[12] Faes S,Dormond O.PI3K and AKT:Unfaithful partners in cancer[J].Int J Mol Sci,2015,16(9):21138-21152.
[13] King D,Yeomanson D,Bryant HE.PI3King the Lock:Targeting the PI3K/Akt/mTOR Pathway as a novel therapeutic strategy in neuroblastoma[J].J Pediatr Hematol Oncol,2015,37(4):245-251.
[14] Jha SK,Jha NK,Kar R,et al.p38 MAPK and PI3K/AKT signalling cascades in Parkinson's disease[J].Int J Mol Cell Med,2015,4(2):67-86.
[15] Kim DY,Rhee I,Paik J.Metabolic circuits in neural stem cells[J].Cell Mol Life Sci,2014,71(21):4221-4241.
[16] Huang W,Zhao Y,Zhu X,et al.Fluoxetine upregulates phosphorylated-AKT and phosphorylated-ERK1/2 proteins in neural stem cells:evidence for a crosstalk between AKT and ERK1/2 Pathways[J].J Mol Neurosci,2013,49(2):244-249.
[17] Zhang Q,Liu G,Wu Y,et al.BDNF promotes EGF-Induced proliferation and migration of human fetal neural stem/progenitor cells via the PI3K/Akt pathway[J].Molecules,2011,16(12):10146-10156.
[18] Li X,Zhang J,Zhu X,Wang P,et al.Progesterone reduces inflammation and apoptosis in neonatal rats with hypoxic ischemic brain damage through the PI3K/Akt pathway[J].Int J Clin Exp Med,2015,8(5):8197-8203.

Effects of lithium chloride on the activity of PI3K/Akt signaling pathway in hypoxic neural stem cells

氯化锂对缺氧后神经干细胞PI3K/Akt信号通道活性的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 7

Recommended Articles

Metrics

[1]	ZHOU Li-xia, CAI Dong, CHEN You-ping. Effect of melatonin regulating Akt/mTOR signal pathway on intestinal barrier function in neonatal rats with necrotizing enterocolitis [J]. Chinese Journal of Child Health Care, 2022, 30(6): 622-626.
[2]	WU Shao-hui, HUANG Xiao-dong, XU Zhi-hang. Analysis of the relationship between the expression of TLRs signaling pathway and inflammatory factors and recurrent respiratory tract infection [J]. journal1, 2021, 29(2): 205-208.
[3]	HE Xue-jia, ZHU Wei-wei, BI Mei-rong. Research progress on the mechanism of gut microbiota participating in airway hyperresponsiveness of allergic asthma through short-chain fatty acids [J]. journal1, 2020, 28(4): 431-434.
[4]	ZHANG Hui, WANG Xin-li, HAN Tong-yan, XING Yan, PIAO Mei-hua. Relationship between homocysteine and insulin sensitivity in rats with intrauterine growth restriction and its possible mechanism [J]. journal1, 2020, 28(2): 152-155.
[5]	CUI Ying, LUAN Zuo, YANG Yin-xiang, WANG Cai-ying, GU Gui-xiong.. Effect of granulocyte-colony stimulating factor on survival of implanted human neural stem cells and the differentiation into oligodendrocytes. [J]. journal1, 2014, 22(2): 149-152.
[6]	WANG Yu, YIN Xiao-juan.. Cultivation, differentiation and identification in vitro of the neural stem cells from neonatal rat. [J]. journal1, 2014, 22(1): 31-34.
[7]	WANG Yu,YIN Xiao-juan. Lithium-mediate influence on stem cells in vitro culture in neonate rat in anaerobic condition [J]. journal1, 2013, 21(10): 1045-1048.