硝酸甘油耐受大鼠主动脉差异microRNA筛选与生物信息学分析

doi:10.12092/j.issn.1009-2501.2018.10.001

中国临床药理学与治疗学 ›› 2018, Vol. 23 ›› Issue (10): 1081-1087.doi: 10.12092/j.issn.1009-2501.2018.10.001

• 基础研究 • 下一篇

硝酸甘油耐受大鼠主动脉差异microRNA筛选与生物信息学分析

曾振华¹，刘建新²，曹春芽²，吴卫华²

¹湖南医药学院生物医学研究中心，怀化 418000，湖南；²湖南医药学院药学院，怀化 418000，湖南

收稿日期:2018-05-30 修回日期:2018-06-25 出版日期:2018-10-26 发布日期:2018-10-25
通讯作者: 吴卫华，男，博士，副教授，硕士生导师，研究方向：心血管药理学。 Tel：0745-2381210 E-mail：wwh_rayan@163.com
作者简介:曾振华，女，硕士，实习研究员，研究方向：心血管药理学。 Tel：0745-2380023 E-mail：18814099612@163.com
基金资助:
湖南省教育厅科学研究项目（17B188）；湖南医药学院青年优秀科研人才支持计划（2013-85）

Screening and bioinformatics analysis of differential microRNA in nitroglycerin-tolerant rat aorta

ZENG Zhenhua¹, LIU Jianxin², CAO Chunya², WU Weihua²

¹Biomedical Research Center, Hunan University of Medicine, Huaihua 418000, Hunan, China; ²School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, Hunan, China

Received:2018-05-30 Revised:2018-06-25 Online:2018-10-26 Published:2018-10-25

摘要/Abstract

摘要：

目的: 筛选硝酸甘油耐受大鼠主动脉差异表达的microRNA（miRNA），并分析预测差异miRNA调控的靶基因及功能。方法: SD大鼠皮下注射给予硝酸甘油连续3 d造成硝酸甘油耐受模型，胸主动脉提取RNA后，应用miRNA测序技术筛选差异miRNA，并用qRT-PCR进行验证。利用生物信息学方法预测差异表达miRNA调控的靶基因，分析靶基因富集的基因功能（gene ontology，GO）和信号通路（pathway）。结果: 与对照组比较，硝酸甘油耐受组共有28个miRNA表达发生显著变化，其中16个为已知miRNA，其中12个上调，4个下调（P<0.05）。qRT-PCR检测了miR-487b-3p和miR-503-5p，变化趋势与测序结果一致。生物信息学分析显示，差异miRNA的靶基因主要富集于蛋白结合和代谢途径。结论: 硝酸甘油耐受大鼠主动脉miRNA表达明显变化，这些差异miRNA通过调控靶基因参与硝酸甘油耐受发生。

关键词: 硝酸甘油, 耐受, microRNA, 生物信息学

Abstract:

AIM: To screen the differential expression of microRNA (miRNA) in nitroglycerin-tolerant rat aorta and to analyze their target genes and function. METHODS: Sprague Dawley (SD) rats were treated with nitroglycerin (100 mg·kg^-1·time^-1 for 3 days, three times a day, s.c). Then, miRNA sequencing technology and qRT-PCR were used to find differential miRNAs involved in nitroglycerin-tolerant rats. Furthermore, bioinformatics analysis was performed to predict target genes regulated by these differential miRNAs, and to analyze the enriched gene ontology (GO) and signaling pathway. RESULTS: Compared with control group, the expression of 28 miRNAs changed significantly in nitroglycerin-tolerant group, of which 16 were known. 12 up-regulated and 4 down-regulated were obtained among the 16 miRNAs. The expression of miR-487b-3p and miR-503-5p were detected by qRT-PCR showed the similar trend as well as the sequencing results. Bioinformatics analysis showed that the target genes of differential miRNAs were mainly enriched in protein binding and metabolic pathways. CONCLUSION: The expression of miRNA in nitroglycerin-tolerant rat aorta changes significantly, and these differential miRNAs are involved in the development of nitroglycerin tolerance by regulating their target genes.

Key words: nitroglycerin, tolerance, microRNA, bioinformatics

中图分类号:

R965.2

曾振华，刘建新，曹春芽，吴卫华. 硝酸甘油耐受大鼠主动脉差异microRNA筛选与生物信息学分析[J]. 中国临床药理学与治疗学, 2018, 23(10): 1081-1087.

ZENG Zhenhua, LIU Jianxin, CAO Chunya, WU Weihua. Screening and bioinformatics analysis of differential microRNA in nitroglycerin-tolerant rat aorta[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(10): 1081-1087.

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硝酸甘油耐受大鼠主动脉差异microRNA筛选与生物信息学分析

Screening and bioinformatics analysis of differential microRNA in nitroglycerin-tolerant rat aorta

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