Determination of aconitine concentration in rats plasma by LC-MS/MS

Abstract

Abstract: AIM: To establish an LC-MS/MS method for determining the concentration of aconitine in rats plasma. METHODS: Verapamil was used as internal standard, the plasma samples extracted by MTBE were separated on a HyPURITY Cyano column (150 mm × 2.1 mm, 5 μm) with mobile phase contained acetonitrile and 10 mmol/L ammonium formate (70∶30, V/V) at flow rate of 0.30 mL/min. The electrospray ionization (ESI source) in positive ion multiple reaction monitoring (MRM) were used for scanning analysis, then, aconitine and verapamil ion selective channels were: m/z 646.4→586.4 and 455.2→164.9, respectively. RESULTS: The linear range for aconitine was 9.3-2390 pg/mL. The lower limit of quantitation was 9.3 pg/mL, and the relative standard deviation of intra-day and inter-day were less than 15%. CONCLUSION: The established method is suitable for pharmacokinetic study of aconitine.

Key words: LC-MS/MS, Aconitine, Pharmacokinetics

CLC Number:

R969.1

WANG Zhi-qi^1,2, JING Xian³, ZENG Rong^1,2, CHEN Yao³, TAN Zhi-rong³. Determination of aconitine concentration in rats plasma by LC-MS/MS[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(4): 371-375.

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