Clone and analysis of splice variants of interleukin 7 in cancer cell lines

Abstract

Abstract: AIM: To clone and select the splice variants in open reading frame of interleukin 7 from cancer cell lines. METHODS: Reverse transcription- polymerase chain reaction (RT- PCR) was used for investigating the expression of IL-7. According to IL-7 gene cDNA, the primers were designed and synthesized, then the splice variants of IL-7 in human cancer cell lines was identified, cloned into vector and sequenced. RESULTS: IL-7 mRNA can be detected in Hepatic cancer cells and Colon cancer cells. A number of new bands of IL-7 were obtained from cultured cancer cell lines, which lack of different exons. By analyzing the splice variants, we found 5 of them in open reading frame of IL-7. CONCLUSION: Splice variants of IL-7 are expressed in many tumor cell lines. Further investigation of the Splice variants can provide a new research direction for cancer patient immune disorders and clinical immunotherapy.

Key words: Interleukin 7, Cloning, Sequence analysis, Tumor cells, Splice variants

CLC Number:

R965.2

FENG Feng-lan¹, PENG Yu-yu¹, PAN De-shun¹, LI Xiao-bo², JIN Xiao-bao², ZHU Jia-yong². Clone and analysis of splice variants of interleukin 7 in cancer cell lines[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(4): 367-370.

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