Jujuboside A attenuates LPS-induced microglial activation and provides neuroprotection

doi:10.12092/j.issn.1009-2501.2018.02.002

Abstract

Abstract:

AIM: To investigate the inhibitory effects of Jujuboside A (Ju A) on LPS induced inflammation mediators in BV-2 microglial cells, and the neuroprotective effect of Ju A in neuronal injury. METHODS: BV-2 cells were pre-treated with Ju A, and then stimulated by LPS. The cell viabilities were measured by MTT assay, the levels of NO were measured by Griess assay, the concentrations of TNF-alfa, IL-1beta were detected by using ELISA, the contents of ROS were detected by DCFH-DA probe method, and the effect of BV-2 conditioned medium on survival rate of HT-22 cells was detected by MTT. RESULTS:Ju A had no significant effect on cell viability within tested concentrations.Ju A significantly inhibited the production of NO, TNF-alfa, IL-1beta and ROS. BV-2 conditioned medium intervened by Ju A inhibited the neuroinflammation on HT-22 hippocampal neuron cells. CONCLUSION: Ju A inhibits LPS-induced microglia activation and neuroinflammation, which mediates its neuroprotective effect on neuronal injury.

Key words: Jujuboside A, microglial cell, hippocampal neurons, neuroinflammation, neuroprotective

CLC Number:

R744
R965.2

ZHANG Rui, LI Bingjie, LI Qinglin. Jujuboside A attenuates LPS-induced microglial activation and provides neuroprotection[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(2): 126-131.

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