Effects of scopolamine on expression of p-CREB and c-Fos in amygdala nucleus in rats with recurrence of conditioned place preference induced by morphine

Abstract

Abstract: AIM: To dectect the effects of scopolamine on expression of phospho-cAMP response element binding protein (p-CREB) and c-Fos in amygdala nucleus (Amy) in rats with recurrence of conditioned place preference(CPP) induced by morphine. METHODS: Morphine was administered by subcutaneous injection at gradually increasing dose(from 10 mg/kg to 60 mg/kg) for 6 days to establish CPP model. From d 7, the rats were administered saline instead of morphine for 10 days to induce CPP extinction. The rats were given a single priming injection of morphine (4 mg/kg) to reinstate the CPP model, some rats were treated by i. p. scopolamine (1, 2, 3 mg/kg) prior to priminging injection of morphine. The expression of p-CREB and c-Fos in Amy were assayed by immunohistochemistry in the phase of reoccurrence of CPP. RESULTS: After priming injection of morphine 4 mg/kg, the time spent on the drug paired side was significantly reduced because of the treatment with scopolamine, compared with that in the morphine group(P<0.05). Compared with the morphine group, scopolamine decreased the expression of p-CREB and c-Fos of Amy in rats (P<0.05). CONCLUSION: The effects of scopolamine inhibiting morphine CPP reccurrence were probably related to its inhibitory effect on p-CREB and c-Fos expression of Amy in rat.

Key words: morphine, conditioned place preference, cAMP response element binding protein, c-Fos, amygdala nucleus

CLC Number:

R965.2

ZHAO Yong-na, LI Xiao-hong, FANG Zheng-mei, LI Shun-ying, SHAO Xiao-xia. Effects of scopolamine on expression of p-CREB and c-Fos in amygdala nucleus in rats with recurrence of conditioned place preference induced by morphine[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(1): 89-93.

References

[1] Pittenger C, Huang YY, Paletzki RF, et al. Reversible inhibition of CREB ATF transcription actors in region CA1 of the dorsal hippocampus disrupts hippocampus-dependent spatial memory[J]. Neuron, 2002, 34(3): 447-462.
[2] Nestler EJ, Aghajanian GK. Molecular and cellular basis of addiction[J]. Science, 1997, 278(5335): 58-63.
[3] Tolliver BK, Sganga MW, Sharp FR. Suppression of c-fos induction in the nucleus accumbens prevents acquisition but not expression of morphine- conditioned place preference[J]. Eur J Neurosci, 2000, 12(9): 3399-3408.
[4] Urani A, Private A. The modulation by neurosteroids of the scopolamine induced learning impairment in mice involves an interaction with sigmal receptors[J]. Brain Research, 1998, 799(1): 64-77.
[5] Mueller D, Stewart J. Cocaine-induced conditioned place preference: reinstatement by priming injections of cocaine after extinction[J]. Behave Brain Res, 2000, 115(1): 39-47.
[6] Heidbreder CA, Shppenberg TS. Evidence for an involvement of muscarinic cholinergic systems in the induction but not expression of behavioral sensitization to cocaine[J]. Synapse, 1996, 24(2): 182-192.
[7] 谷建平, 丛斌, 朱桂云, 等. 褪黑素对吗啡依赖大鼠复吸行为的抑制作用[J]. 中国药物依赖杂志, 2004,13(3): 187-190.
[8] Paxinos G, Watson GC. The rat brain in stereotaxic coordinates[M]. New York:Academic, 1982.
[9] 段新. 纳洛酮和加兰他敏对东莨菪碱所致大鼠空间学习障碍的作用[D]. 汕头大学, 2003.
[10] 杨国栋, 徐坤, 罗群, 等. 东莨菪碱对海洛因依赖者脱毒治疗的临床观察[J]. 中华医学杂志, 1996, 76(2): 141-147.
[11] 张世仪, 吴馥梅, 蔡景霞. 促智药物的研究[A] 韩太真, 吴馥梅, 主编. 学习与记忆的神经生物学. 北京:北京医科大学、中国协和医科大学联合出版社,1998:405.
[12] 李新旺, 郑希耕, 杨晓燕, 等. 东莨菪碱和毒扁豆碱对吗啡训练大鼠空间学习的影响[J]. 中国药物依赖性杂志, 2002, 11(4): 254-256.
[13] 李国海, 黄明生. 东莨菪碱消除吗啡所致条件位置偏爱的形成[J]. 华西医学, 2001, 16(1): 33-34.
[14] 谌红献, 张瑞玲, 郝伟. 东莨菪碱对SD 大鼠吗啡位置偏爱的影响[J]. 中国行为医学科学杂志, 2001, 10(4): 289-293.
[15] Mclntyre CK, Ragozzino ME, Gold PE, et al. Intraamygdala infusions of scopolamine impair performance on a conditioned place preference task but not a spatial radial maze task[J]. Brain Res, 1998, 95: 219-226.
[16] Morgan JI, Curran T. Stimulus-transcripition coupling in the nervous system: role of cellular immediate-early genes[J]. Trends Neurosci, 1989, 12(11): 459-462.