Psychophysiological observation of propofol addiction in rats

doi:10.12092/j.issn.1009-2501.2019.06.002

Abstract

Abstract:

AIM: To observe the addiction action of propofol on rats and explore the possible psychophysiological characteristics of the model rats. METHODS: Thirty-three adult male SD rats were divided into two groups randomly, i.e. Propofol Group (n=18) and Control Group (n=15). After a week of adaptive feeding, an 11-day conditioned place preference (CPP) experiment, including pretest stage, training stage and posttest stage was conducted in all rats.After CCP experiment, the electric activities from addiction-related nuclei accumbens nucleus core (Acbc) and basolateral amygdala (BLA) were extracellularly recorded, and physiological functions of electrocardiogram (ECG), electromyogram (EMG) of respiratory muscles and body temperature were simultaneously observed. The changes of electrical activities in Acbc and BLA, and physiological functions were observed after intraperitoneal injection of NS and propofol. RESULTS:(1) There was an increase of the time in the drug box for the propofol group rats (P<0.01), and an increased difference between posttest and pretest in comparison with control group rats (P<0.05). (2) The firing rate in Acbc and BLA in propofol group rats (n=10) was significantly higher than that in the control group rats (n=5) (Acbc: P<0.01, BLA: P<0.05). (3) An increased Acbc/BLA ratio of firing rate in propofol group rats was seen in comparison with control group rats (P<0.05). (4) No significant changes of firing rate were observed after injecting propofol but the respiratory rate and body temperature in propofol group rats decreased significantly (P<0.05, n=8). CONCLUSION: These results indicate that the short-term repeated administration of small dose propofol may lead to addiction, which is possibly related to the changes in reward-punishment system balance in the brain of rats.

Key words: propofol, addiction, conditioned place preference experiment, psychophysiology

CLC Number:

XU Wenting, YANG Liqin, ZHANG Zhongnan, LIANG Jun, JIANG Nan, JIANG Miao, GUO Wenjun, WANG Mengya. Psychophysiological observation of propofol addiction in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(6): 608-614.

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