Effects of Dexamethasoneon apoptosis of HepG2 cells induced by Cisplatin and expression of Bcl-2 and Caspase-3

Abstract

Abstract: AIM: To investigate the mechanism of Dexamethasone (DEX) on apoptosis of HepG2 cells induced by Cisplatin (DDP) and the expression of Bcl-2 and Caspase-3. METHODS: HepG2 and DDP were co-cultured, a serials of concentrations DEX were added into it, the expression of mRNA and protein of Bcl-2 and Capase-3 were analyzed separately by RT-PCR and Western blot,and the percentage of cell apoptosis was analyzed by FACS. RESULTS: With DEX increases in concentration, the expression of Bcl-2 was increased and expression of Caspase-3 was decreased correspondently, the rate of apoptosis of HepG2 descends clearly.CONCLUSION: DEX restrains apoptosis of HepG2 cells induced by Cisplatin (DDP) partly through Bcl-2 anti-apoptosis pathways, and Caspase-3 plays an important role in regulating the cell apoptosis.

Key words: Dexamethasone, Cisplatin, HepG2 cells, Apoptosis

CLC Number:

R965.2

TAN Jun, RUAN Bing, ZHOU Mi, GAO Guo-sheng. Effects of Dexamethasoneon apoptosis of HepG2 cells induced by Cisplatin and expression of Bcl-2 and Caspase-3[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(10): 1118-1123.

References

[1] Jemal A, Siegel R, Ward E, et al.Cancer statistics[J]. CA Cancer J Clin, 2009, 59(4): 225-249.
[2] Nagai H, Sumino Y.Therapeutic strategy of advanced hepatocellular carcinoma by using combined intra-arterial chemotherapy[J]. Recent Pat Anticancer Drug Discov, 2008, 3(3): 220-226.
[3] Musso M, Scalone R, Bonanno V, et al.Palonosetron (Aloxi) and dexamethasone for the prevention of acute and delayed nausea and vomiting in patients receiving multiple-day chemotherapy[J]. Support Care Cancer, 2009, 17(2): 205-209.
[4] Qian YH, Xiao Q, Chen H, et al.Dexamethasone inhibits camptothecin-induced apoptosis in C6-glioma via activation of Stat5/Bcl-xL pathway[J]. Biochim Biophys Acta, 2009, 1793(5): 764-771.
[5] Chipuk JE, Moldoveanu T, Llambi F, et al.The BCL-2 family reunion[J]. Mol Cell, 2010, 37(3): 299-310.
[6] Patel MP, Masood A, Patel PS, et al.Targeting the Bcl-2[J]. Curr Opin Oncol, 2009, 21(6): 516-523.
[7] Gao CN, Suchida T.Activation of caspases in p53-induced transactivation-independent apoptosis[J]. Jpn J Cancer Res, 1999, 90(2): 180-187.
[8] Satoh K, Kaneko K, Hirota M, et al.The pattern of CPP32/caspase-3 expression reflects the biological behavior of the human pancreatic duct cell tumors[J]. Pancreas, 2000, 21(4): 352-357.
[9] Fuertes MA, Alonso C, Pérez JM.Biochemical modulation of cisplatin mechanisms of action: enhancement of antitumor activity and circumvention of drug resistance[J]. Chem Rev, 2003, 103(3):645-662.
[10] Anai S, Sakamoto N, Sakai Y, et al.Dual targeting of Bcl-2 and VEGF: A potential strategy to improve therapy for prostate cancer[J]. Urol Oncol, 2011, 29(4): 421-429.
[11] Han D, Denison MS, Tachibana H, et al.Effects of estrogenic compounds on immunoglobulin production by mouse splenocytes[J]. Biol Pharm Bull, 2002, 25(10): 1263-1267.

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