Abstract: AIM: Research on the tetrastigma hemsleyanum diels et gilg flavone (sanyeqing),which influences the tumor volume and the expression level of TGFβ₁, PGE₂,COX-2 of LLC tumor bearing C57BL/6 mice, to explore the possible mechanism of reverse tumor immune escape function of radix tetrastigme to tumor-bearing mice.METHODS: LLC tumor bearing C57BL/6 mice models were established by tumor homogenate inoculation method and were randomly divided into normal group, model control group, high dose group of the total flavonoids from radix tetrastigmae, middle dose group of the total flavones from radix tetrastigmae, low dose group of the total flavonoids from radix tetrastigmae. Stripped the tumor tissue and weighed after 14 days continuous administration. The ELISA method was used to detected the TGFβ₁, PGE₂ and COX-2 content in serum.RESULTS: Tumor volume: compared with the model control group, high-dose group, middle-dose group had significant difference in tumor volume (P<0.05). Tumors size in the order: model control group>low-dose group>middle-dose group> high-dose group. TGF-β₁, PGE₂ and COX-2 content in serum: after the experiment, the experimental groups were significantly higher than the normal group (P<0.01), within groups, high-dose group< low-dose group (P<0.05).CONCLUSION: The tetrastigma hemsleyanum diels et gilg flavone high-dose group had a better effect in suppressing tumor growth, the middle dose group didn't play an effective role, And the low-dose group had the most weak effect in suppressing tumor growth. The tumor suppression effect of tetrastigma hemsleyanum diels et gilg flavone may be related to the lower levels of TGF-β₁ expression in peripheral blood, and may decrease the level of PGE₂, COX-2 expression, so as to achieve reverse tumor escape, to achieve the anti-tumor effect.

Key words: tetrastigma hemsleyanum diels et gilg flavone, TGF-β₁, immune escape, COX-2, PGE₂