关键词: 氧化苦参碱, 人三阴性乳腺癌, 上皮间质转化, 侵袭, 生长抑制

Abstract:

AIM: To study the inhibitory effects of oxymatrine on growth and migration of human triple-negative breast cancer MDA-MB-231 cell.  METHODS: CCK-8 assay was used to detect the inhibitory effects of oxymatrine on the growth of MDA-MB-231 cells. Inhibitory effect of oxymatrine on cell cycle of MDA-MB-231 cells was detected by flow cytometry (FCM). Effect of oxymatrine on migration of MDA-MB-231 cells was detected by scratch experimental. RESULTS: Compared with the control group, the growth of MDA-MB-231 cells decreased significantly in a time- and dose-dependent manner after oxymatrine treatment (2, 4, 6, 8, 10, 20 mg/mL for 24, 48 and 72 h). Oxymatrine significantly increased the number of cells in G0/G1 phase and decreased the number of cells in S and G2/M phase（P<0.05）. Transwell showed that oxymatrine treated MDA-MB-231 cells for 12 h significantly inhibited the invasion of MDA-MB-231. The high concentration of oxymatrine significantly up-regulated the expression of E-cadherin and inhibited the expression of Snail-2 mRNA (P<0.01). CONCLUSION: Oxymatrine exerts its antitumor effect by inhibiting the growth and invasion of MDA-MB-231 cells, which plays a potential role on anti-tumor growth and metastasis.

Key words: oxymatrine, triple-negative breast cancer cells, EMT, migration, growth inhibition