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中国临床药理学与治疗学 ›› 2001, Vol. 6 ›› Issue (4): 334-338.

• 基础研究 • 上一篇    下一篇

大豆异黄酮对YAC-1 细胞和小鼠ESC艾氏肉瘤生长的抑制作用

余丽梅, 徐璐2, 姚明辉2   

  1. 遵义医学院药理学教研室, 遵义 563003;
    2复旦大学医学院药理学教研室, 上海 200032
  • 收稿日期:2001-07-14 修回日期:2001-08-12 出版日期:2001-08-26 发布日期:2020-11-30
  • 作者简介:余丽梅, 女, 副教授。姚明辉, 男, 教授, 博士生导师。
  • 基金资助:
    本课题为美国中华医学基金会(CM B) 2001 年资助项目。

Inhibitory effects of isoflavones on growth of YAC-1 T lymphocyte tumor cells of mice in vitro and ehrlich-ascites-tumor of mice in vivo

YU Li-Mei, XU Lu2, YAO Ming-Hui2   

  1. Department of Pharmacology, Zunyi Medical College, Zunyi 563003;
    2Department of Pharmacology, Medical College of Fudan University, Shanghai 200032
  • Received:2001-07-14 Revised:2001-08-12 Online:2001-08-26 Published:2020-11-30

摘要: 目的 观察大豆异黄酮对YAC-1 细胞及小鼠ESC艾氏肉瘤生长的抑制作用及对免疫功能的影响。方法 用MT T 法测定大豆异黄酮对YAC-1 细胞生长的抑制率和小鼠淋巴细胞转化刺激指数;LDH 释放法测定小鼠NK 细胞活性;称重观察肿瘤生长和脾指数和胸腺指数。结果 金雀异黄素和大豆甙元剂量依赖性抑制YAC-1 细胞生长, 并随时间延长作用增强;大豆异黄酮50、100、400 mg·kg-1 灌胃给药均明显升高雌性小鼠脾指数和淋巴细胞转化的刺激指数, 对雄性小鼠则无明显作用;大豆异黄酮100、400 mg·kg-1 明显抑制小鼠ESC 艾氏肉瘤生长, 同时50、100、400 mg·kg-1 均降低异常增高的脾指数, 增加NK 细胞活性。结论 大豆异黄酮明显抑制YAC-1 细胞和小鼠ESC 艾氏肉瘤的生长,同时增强非特异性免疫功能, 并可增强正常雌性小鼠免疫功能。

关键词: 大豆异黄酮, 金雀异黄素, 大豆甙元, 肿瘤, 免疫功能

Abstract: Aim To observe the effect of isoflavones on the growth of YAC-1 cells in vitro and ESC tumor in mice and their immune function. Methods Isof lavones induced inhibitory rate of YAC-1 cells and the proliferation of lymphocy te were assessed by the modif ied MTT assay.NK cell activities were determined by LDH.The anti tumor activity in vivo was observed by weighing the tumor and calculating spleen and thymus indexes.Results The grow th of YAC-1 cells were inhibited by isof lavones in a concentration and time-dependent manner.Isof lavones power ext racted from soybean (50, 100, 400 mg·kg-1, o ral administ ration) could enhance spleen index and ConA-induced proliferation of lymphocyte in female mice, but not in male ones.Isoflavones in the doses of 50, 100, 400 mg·kg-1 could significant ly inhibit spleen index and increase NK cell activi ties.The grow th of tumor was significant ly inhibited in ESC tumor-bearing female mice in the doses of 100, 400 mg·kg-1.Conclusion Isof lavones has inhibitory effect on the growths YAC-1 cells in vi tro and ESC tumor in vivo.Isoflavones not only strengthens the nonspecail immune function of tumor-bearing mice, but also of the normal female mice.

Key words: isoflavones, genistein, daidzein, tumor, immune function

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