普罗布考对受损内皮细胞增殖和功能修复的促进作用1

中国临床药理学与治疗学 ›› 2002, Vol. 7 ›› Issue (5): 401-405.

普罗布考对受损内皮细胞增殖和功能修复的促进作用¹

谢志忠, 杨云波, 陆明辉², 黄红林, 廖端芳

南华大学药物药理研究所, 衡阳 421001

收稿日期:2002-04-16 修回日期:2002-06-11 发布日期:2020-11-26

Effects of probucol on proliferation and function recovery of injured endothelial cells¹

XIE Zhi-Zhong, YANG Yun-Bo, LU Ming-Hui², HUANG Hong-Lin, LIAO Duan-Fang

Institute of Pharmacy &Pharmacology, Nanhua University, Hengyang 421001

Received:2002-04-16 Revised:2002-06-11 Published:2020-11-26
About author:XIE Zhi-Zhong, male, postgraduate, lecturer.LIAO Duan-Fang, male, PhD, prof essor, vi ce-president of Nanhua University,engaged in cardiovascular pharmacology.Tel:86-734-8281308 E-mail:dfliao @hotmail.com.2 Present address:PuLin Pharmaceuti cal Facto
Supported by:
1 Project support ed by the National Natural S cience Foundation of China (№39970847) and the Foundation of Hunan Educative Committee (№JY01C18)

摘要/Abstract

摘要： 目的研究普罗布考对受损内皮细胞增殖和再生内皮细胞功能修复的影响。方法分别采用MTT 比色法和PCNA 免疫组化法测定体外培养的人脐静脉内皮细胞所处增殖状态;以兔胸主动脉血管环对乙酰胆碱舒张反应和内皮细胞分泌NO、PGI2 的能力评价内皮细胞功能状况。结果 LPC 作用24 h可导致内皮细胞增殖显著受抑及分泌NO、PGI2 功能受损, 不同剂量普罗布考(20, 40, 80 μmol·L^-1) 可促进LPC 损伤的内皮细胞增殖(55.5 %, 59.3 %,72.2 % vs 31.7 %, P <0.01), 同时提高血管成型术后兔胸主动脉血管环内皮依赖性舒张功能(66.63 %vs 25.95 %, P <0.01), 并修复LPC 损伤的内皮细胞分泌NO、PGI2 的功能(P <0.01)。结论普罗布考可促进受损内皮细胞增殖和再生内皮细胞功能修复。

关键词: 普罗布考, 再狭窄, 内皮细胞, 细胞增殖

Abstract: AIM: To study the effects of probucol on injured endothelial cells and regenerated endothelium. METHODS: In cultured human umbilical vein endothelial cells (huVECs), various concentration of probucol (20, 40, 80 μmol · L ^-1) were given 30 min after lysophophatidylcholine (LPC, 20 mg ·L ^-1) treatment. MTT assay and PCNA immunohistochemical staining assay were used to evaluate the proliferation of injured huVECs. The function of endothelium was estimated by measuring the relaxation response of PTA rabbits thoracic aorta rings to acetylcholine and by measuring NO and PGI2 release of cultured huVECs. RESULTS: LPC treatment for 24 h obviously inhibited huVECs proliferation and attenuated huVECs release of NO and PGI2 (P <; 0.01).Probucol (20, 40, and 80 μmol·L^-1) promoted the proliferation of injured huVECs with the growth rate from 31.7 % to 55.5 %, 59.3 % and 72.2 % respectively as shown by MTT assay.Probucol also enhanced vascular relaxation response of rabbits thoracic aorta rings to acetylcholine from 25.95 % to 66.63 %and increased NO and PGI2 release of injured huVECs. CONCLUSION: Probucol can promote proliferation and function recovery of impaired endothelial cells.;

Key words: probucol, restenosis, endothelial cell, proliferation

谢志忠, 杨云波, 陆明辉, 黄红林, 廖端芳. 普罗布考对受损内皮细胞增殖和功能修复的促进作用¹[J]. 中国临床药理学与治疗学, 2002, 7(5): 401-405.

XIE Zhi-Zhong, YANG Yun-Bo, LU Ming-Hui, HUANG Hong-Lin, LIAO Duan-Fang. Effects of probucol on proliferation and function recovery of injured endothelial cells¹[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2002, 7(5): 401-405.

参考文献

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普罗布考对受损内皮细胞增殖和功能修复的促进作用¹

Effects of probucol on proliferation and function recovery of injured endothelial cells¹

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